論文 - 詳細
| RRC ID | 11774 |
|---|---|
| 著者 | Noda S, Ichikawa H, Miyoshi H. |
| タイトル | Hematopoietic stem cell aging is associated with functional decline and delayed cell cycle progression. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The molecular mechanisms underlying hematopoietic stem cell (HSC) aging remain to be elucidated. In this study, we investigated age-related changes in the functional and phenotypic properties of murine HSCs. Consistent with previous studies, we found that the number and frequency of CD34(-/low)c-Kit(+)Sca-1(+)lineage marker(-) (CD34(-)KSL) cells, a highly enriched HSC population, significantly increased in old mice, though their repopulating ability was reduced. Continuous bromodeoxyuridine labeling revealed a significant delay in the cell cycle progression of CD34(-)KSL cells in old mice. This delay was also observed in young recipients transplanted with whole bone marrow cells from old mice. When cultured in vitro, CD34(-)KSL cells from old mice showed a greater capacity to give rise to primitive CD48(-)KSL cells with reduced HSC activity. Gene expression profiling identified age-related changes in the expression of several cell cycle regulatory genes, including p21/Cdkn1a and p18/Cdkn2c. These results support the notion that HSC aging is largely regulated by an intrinsic genetic program. |
| 巻・号 | 383(2) |
| ページ | 210-5 |
| 公開日 | 2009-5-29 |
| DOI | 10.1016/j.bbrc.2009.03.153 |
| PII | S0006-291X(09)00655-X |
| PMID | 19345668 |
| MeSH | Animals Cell Cycle* / genetics Cell Proliferation Cells, Cultured Cellular Senescence* / genetics Gene Expression Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / metabolism Hematopoietic Stem Cells / physiology* Mice Mice, Inbred C57BL Oligonucleotide Array Sequence Analysis |
| IF | 2.985 |
| 引用数 | 26 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 実験動物マウス | |