| RRC ID |
11811
|
| Author |
Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H.
|
| Title |
Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
Mouse transgenesis has proven invaluable for analysis of gene function and generation of human disease models. We describe here the development of a pronuclear injection-based targeted transgenesis (PITT) system, involving site-specific integration in fertilized eggs. The system was applied to two different genomic target loci to generate a series of transgenic lines including fluorescent mice, which reproducibly displayed strong, ubiquitous and stable transgene expression. We also demonstrated that knockdown mice could be readily generated by PITT by taking advantage of the reproducible and highly efficient expression system. The PITT system, which circumvents the problem of unpredictable and unstable transgene expression of conventional random-integration transgenic mice, reduces the time, cost and effort needed to generate transgenic mice, and is potentially applicable to both in vivo 'gain-of-function' and 'loss-of-function' studies.
|
| Volume |
38(22)
|
| Pages |
e198
|
| Published |
2010-12-1
|
| DOI |
10.1093/nar/gkq860
|
| PII |
gkq860
|
| PMID |
20880997
|
| PMC |
PMC3001095
|
| MeSH |
Animals
Cell Nucleus
Embryonic Stem Cells / metabolism
Gene Expression
Gene Knockdown Techniques
Gene Targeting / methods*
Injections
Integrases / metabolism
Mice
Mice, Transgenic*
Ovum / metabolism
Recombination, Genetic
Sequence Deletion
Transgenes*
|
| IF |
11.502
|
| Times Cited |
36
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Mice |
RBRC01835 |
