論文 - 詳細
| RRC ID | 11824 |
|---|---|
| 著者 | Fujihara Y, Takato T, Hoshi K. |
| タイトル | Immunological response to tissue-engineered cartilage derived from auricular chondrocytes and a PLLA scaffold in transgenic mice. |
| ジャーナル | Biomaterials |
| Abstract |
The immune response against biomaterials in tissue-engineered constructs could potentially worsen the outcome of tissue regeneration, but immunological reactions between host and donor in tissue-engineered constructs remain to be clarified. In the present study, we syngenically transplanted tissue-engineered cartilage constructs consisting of C57BL/6 mice auricular chondrocytes and poly-l-lactic acid scaffolds (MW:200,000) into EGFP transgenic mice of C57BL/6 background, and evaluated the response by the localization of donor-derived and host-derived cells, the latter of which were distinguished by the presence of EGFP. While donor-derived cells constituted the areas of regenerated cartilage, host-derived cells were increased in number for the initial two weeks, and then decreased and excluded to non-cartilage areas thereafter. Furthermore, EGFP positivity was mostly co-localized with that of F4/80, suggesting most of the host-derived cells in the tissue-engineered constructs could be macrophages. Immunohistochemical staining of the tissue-engineered cartilage constructs revealed expression of factors related to immune privilege in chondrocytes, such as macrophage migration inhibitory factor (MIF), fas ligand (FasL) and others. Co-culture of chondrocytes and macrophages in vitro increased the expression of MIF and FasL in the chondrocytes, suggesting that chondrocytes in tissue-engineered cartilage constructs could regulate the actions of host-derived macrophages by expressing factors related to immune privilege. |
| 巻・号 | 31(6) |
| ページ | 1227-34 |
| 公開日 | 2010-2-1 |
| DOI | 10.1016/j.biomaterials.2009.10.053 |
| PII | S0142-9612(09)01174-0 |
| PMID | 19913296 |
| MeSH | Animals Bioartificial Organs* Biocompatible Materials / chemistry Cartilage / growth & development Cartilage / immunology* Cartilage / transplantation* Cells, Cultured Chondrocytes / immunology* Chondrocytes / transplantation* Ear Auricle / cytology* Ear Auricle / immunology Extracellular Matrix / chemistry Humans Lactic Acid / chemistry Materials Testing Mice Mice, Inbred C57BL Mice, Transgenic Polyesters Polymers / chemistry Tissue Engineering / methods* |
| IF | 10.317 |
| 引用数 | 34 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00267 |
| ヒト・動物細胞 | Mm1(RCB0748) |