RRC ID 11826
著者 Nishio N, Ito S, Suzuki H, Isobe K.
タイトル Antibodies to wounded tissue enhance cutaneous wound healing.
ジャーナル Immunology
Abstract The wound repair process is a highly ordered sequence of events that encompasses haemostasis, inflammatory cell infiltration, tissue regrowth and remodelling. Wound healing follows tissue destruction so we hypothesized that antibodies might bind to wounded tissues, which would facilitate the engulfment of damaged tissues by macrophages. Here, we show that B cells, which produce antibodies to damaged tissues, are engaged in the process of wound healing. Splenectomy delayed wound healing, and transfer of spleen cells into splenectomized mice recovered the delay in wound healing. Furthermore, wound healing in splenectomized nude mice was also delayed. Transfer of enriched B220(+) cells by magnetic beads accelerated wound healing in splenectomized mice. We detected immunoglobulin G1 (IgG1) binding to wounded tissues by using fluorescein isothiocyanate-labelled anti-IgG1 6-24 hr after wounding. Splenectomy reduced the amount of IgG1 binding to wounded tissues. Immunoblotting studies revealed several bands, which were reduced by splenectomy. Using immunoprecipitation with anti-IgG bound to protein G we found that the intensity of several bands was lower in the serum from splenectomized mice than in that from sham-operated mice. These bands were matched to myosin IIA, carbamoyl-phosphate synthase, argininosuccinate synthase, actin and alpha-actinin-4 by liquid chromatography tandem mass spectrometry analysis.
巻・号 128(3)
ページ 369-80
公開日 2009-11-1
DOI 10.1111/j.1365-2567.2009.03119.x
PII IMM3119
PMID 20067537
PMC PMC2770685
MeSH Actinin / immunology Actinin / metabolism Actins / immunology Actins / metabolism Adoptive Transfer Animals Antigen-Antibody Complex / immunology Antigen-Antibody Complex / metabolism* Argininosuccinate Synthase / immunology Argininosuccinate Synthase / metabolism B-Lymphocytes / immunology B-Lymphocytes / metabolism* B-Lymphocytes / pathology B-Lymphocytes / transplantation Carbamoyl-Phosphate Synthase (Ammonia) / immunology Carbamoyl-Phosphate Synthase (Ammonia) / metabolism Cells, Cultured Female Immunoglobulin G / immunology Immunoglobulin G / metabolism* Leukocyte Common Antigens / biosynthesis Mice Mice, Inbred C57BL Mice, Nude Nonmuscle Myosin Type IIA / metabolism Skin / immunology Skin / injuries Skin / metabolism* Spleen / pathology Spleen / surgery Splenectomy Wound Healing / immunology*
IF 5.016
引用数 31
WOS 分野 IMMUNOLOGY
リソース情報
実験動物マウス RBRC00267