論文 - 詳細
| RRC ID | 11836 |
|---|---|
| 著者 | Bancos S, Baglole CJ, Rahman I, Phipps RP. |
| タイトル | Induction of heme oxygenase-1 in normal and malignant B lymphocytes by 15-deoxy-Delta(12,14)-prostaglandin J(2) requires Nrf2. |
| ジャーナル | Cell Immunol |
| Abstract |
Heme-oxygenase-1 (HO-1) is induced in response to oxidative stress and is believed to be a cytoprotective and anti-inflammatory enzyme. It is unknown whether normal or malignant human B-lineage cells express HO-1. 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is an interesting electrophilic lipid mediator able to increase oxidative stress in B cells. Here, we tested normal and malignant human B-lineage cells for their ability to express HO-1 in response to 15d-PGJ(2), as well as the signaling pathways required for HO-1 expression. 15d-PGJ(2) potently induced HO-1 protein expression in normal and malignant B cells. Malignant B cells exhibited a greater induction of HO-1 protein compared to normal B lymphocytes. Using siRNA directed against the transcription factor Nrf2 and B cells isolated from Nrf2-deficient mice, we show that HO-1 induction by 15d-PGJ(2) is dependent on Nrf2. These results show that, compared to normal B lymphocytes, malignant B cells have a greater capacity to increase their HO-1 protein levels in response to 15d-PGJ(2). We speculate that the ability to highly express HO-1 by malignant B cells could confer a survival advantage. |
| 巻・号 | 262(1) |
| ページ | 18-27 |
| 公開日 | 2010-1-1 |
| DOI | 10.1016/j.cellimm.2009.12.003 |
| PII | S0008-8749(09)00223-8 |
| PMID | 20064636 |
| PMC | PMC3117258 |
| MeSH | Animals B-Lymphocytes / immunology B-Lymphocytes / metabolism* Cells, Cultured Gene Expression Regulation, Enzymologic Heme Oxygenase-1 / metabolism* Humans Leukemia, B-Cell / immunology Leukemia, B-Cell / metabolism* Mice Mice, Inbred C57BL Mice, Knockout NF-E2-Related Factor 2 / deficiency NF-E2-Related Factor 2 / genetics NF-E2-Related Factor 2 / metabolism* Prostaglandin D2 / analogs & derivatives* Prostaglandin D2 / immunology RNA, Small Interfering / genetics Transcription, Genetic |
| IF | 4.078 |
| 引用数 | 17 |
| WOS 分野 | IMMUNOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 実験動物マウス | RBRC01390 |