| RRC ID |
11836
|
| 著者 |
Bancos S, Baglole CJ, Rahman I, Phipps RP.
|
| タイトル |
Induction of heme oxygenase-1 in normal and malignant B lymphocytes by 15-deoxy-Delta(12,14)-prostaglandin J(2) requires Nrf2.
|
| ジャーナル |
Cell Immunol
|
| Abstract |
Heme-oxygenase-1 (HO-1) is induced in response to oxidative stress and is believed to be a cytoprotective and anti-inflammatory enzyme. It is unknown whether normal or malignant human B-lineage cells express HO-1. 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is an interesting electrophilic lipid mediator able to increase oxidative stress in B cells. Here, we tested normal and malignant human B-lineage cells for their ability to express HO-1 in response to 15d-PGJ(2), as well as the signaling pathways required for HO-1 expression. 15d-PGJ(2) potently induced HO-1 protein expression in normal and malignant B cells. Malignant B cells exhibited a greater induction of HO-1 protein compared to normal B lymphocytes. Using siRNA directed against the transcription factor Nrf2 and B cells isolated from Nrf2-deficient mice, we show that HO-1 induction by 15d-PGJ(2) is dependent on Nrf2. These results show that, compared to normal B lymphocytes, malignant B cells have a greater capacity to increase their HO-1 protein levels in response to 15d-PGJ(2). We speculate that the ability to highly express HO-1 by malignant B cells could confer a survival advantage.
|
| 巻・号 |
262(1)
|
| ページ |
18-27
|
| 公開日 |
2010-1-1
|
| DOI |
10.1016/j.cellimm.2009.12.003
|
| PII |
S0008-8749(09)00223-8
|
| PMID |
20064636
|
| PMC |
PMC3117258
|
| MeSH |
Animals
B-Lymphocytes / immunology
B-Lymphocytes / metabolism*
Cells, Cultured
Gene Expression Regulation, Enzymologic
Heme Oxygenase-1 / metabolism*
Humans
Leukemia, B-Cell / immunology
Leukemia, B-Cell / metabolism*
Mice
Mice, Inbred C57BL
Mice, Knockout
NF-E2-Related Factor 2 / deficiency
NF-E2-Related Factor 2 / genetics
NF-E2-Related Factor 2 / metabolism*
Prostaglandin D2 / analogs & derivatives*
Prostaglandin D2 / immunology
RNA, Small Interfering / genetics
Transcription, Genetic
|
| IF |
4.078
|
| 引用数 |
17
|
|
WOS 分野
|
IMMUNOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC01390 |
