| RRC ID |
11852
|
| 著者 |
Fujishima Y, Nishiumi S, Masuda A, Inoue J, Nguyen NM, Irino Y, Komatsu M, Tanaka K, Kutsumi H, Azuma T, Yoshida M.
|
| タイトル |
Autophagy in the intestinal epithelium reduces endotoxin-induced inflammatory responses by inhibiting NF-κB activation.
|
| ジャーナル |
Arch Biochem Biophys
|
| Abstract |
Autophagy is a lysosomal degradation pathway that is essential for survival, differentiation, development and homeostasis. There is growing evidence that impaired autophagy leads to the pathogenesis of diverse diseases. However, the role of autophagy in intestinal epithelium is not clearly understood, although previous studies have pointed out the possibility for the relationships of autophagy with bowel inflammation. In this study, we investigated the involvement of autophagy in intestinal epithelium with inflammatory responses. We generated the mice with a conditional deletion of Atg7, which is one of the autophagy regulated gene, in intestinal epithelium. In Atg7-deficient small intestinal epithelium, LPS-induced production of TNF-α and IL-1β mRNA was enhanced in comparison to the control small intestinal tissues. In addition, the degree of LPS-induced activation of NF-κB was promoted in Atg7-deficient intestinal epithelium. These results demonstrate that autophagy can attenuate endotoxin-induced inflammatory responses in intestinal epithelium resulting in the maintenance of intestinal homeostasis.
|
| 巻・号 |
506(2)
|
| ページ |
223-35
|
| 公開日 |
2011-2-15
|
| DOI |
10.1016/j.abb.2010.12.009
|
| PII |
S0003-9861(10)00506-0
|
| PMID |
21156154
|
| MeSH |
Animals
Autophagy / drug effects
Autophagy / genetics
Autophagy / physiology*
Autophagy-Related Protein 7
Base Sequence
DNA / metabolism
DNA Primers / genetics
Inflammation / genetics
Inflammation / metabolism
Inflammation / pathology*
Inflammation / prevention & control
Interleukin-1beta / genetics
Intestinal Mucosa / drug effects
Intestinal Mucosa / metabolism*
Intestinal Mucosa / pathology*
Intestine, Small / drug effects
Intestine, Small / metabolism
Intestine, Small / pathology
Lipopolysaccharides / toxicity*
Mice
Mice, Knockout
Mice, Transgenic
Microtubule-Associated Proteins / deficiency
Microtubule-Associated Proteins / genetics
NF-kappa B / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Tumor Necrosis Factor-alpha / genetics
|
| IF |
3.391
|
| 引用数 |
57
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00806 |
