RRC ID 11852
Author Fujishima Y, Nishiumi S, Masuda A, Inoue J, Nguyen NM, Irino Y, Komatsu M, Tanaka K, Kutsumi H, Azuma T, Yoshida M.
Title Autophagy in the intestinal epithelium reduces endotoxin-induced inflammatory responses by inhibiting NF-κB activation.
Journal Arch. Biochem. Biophys.
Abstract Autophagy is a lysosomal degradation pathway that is essential for survival, differentiation, development and homeostasis. There is growing evidence that impaired autophagy leads to the pathogenesis of diverse diseases. However, the role of autophagy in intestinal epithelium is not clearly understood, although previous studies have pointed out the possibility for the relationships of autophagy with bowel inflammation. In this study, we investigated the involvement of autophagy in intestinal epithelium with inflammatory responses. We generated the mice with a conditional deletion of Atg7, which is one of the autophagy regulated gene, in intestinal epithelium. In Atg7-deficient small intestinal epithelium, LPS-induced production of TNF-α and IL-1β mRNA was enhanced in comparison to the control small intestinal tissues. In addition, the degree of LPS-induced activation of NF-κB was promoted in Atg7-deficient intestinal epithelium. These results demonstrate that autophagy can attenuate endotoxin-induced inflammatory responses in intestinal epithelium resulting in the maintenance of intestinal homeostasis.
Volume 506(2)
Pages 223-35
Published 2011-2-15
DOI 10.1016/j.abb.2010.12.009
PII S0003-9861(10)00506-0
PMID 21156154
MeSH Animals Autophagy / drug effects Autophagy / genetics Autophagy / physiology* Autophagy-Related Protein 7 Base Sequence DNA / metabolism DNA Primers / genetics Inflammation / genetics Inflammation / metabolism Inflammation / pathology* Inflammation / prevention & control Interleukin-1beta / genetics Intestinal Mucosa / drug effects Intestinal Mucosa / metabolism* Intestinal Mucosa / pathology* Intestine, Small / drug effects Intestine, Small / metabolism Intestine, Small / pathology Lipopolysaccharides / toxicity* Mice Mice, Knockout Mice, Transgenic Microtubule-Associated Proteins / deficiency Microtubule-Associated Proteins / genetics NF-kappa B / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Tumor Necrosis Factor-alpha / genetics
IF 3.559
Times Cited 46
Mice GFP-LC3#53