RRC ID 11863
Author Lee JE, Oh HA, Song H, Jun JH, Roh CR, Xie H, Dey SK, Lim HJ.
Title Autophagy regulates embryonic survival during delayed implantation.
Journal Endocrinology
Abstract Delayed implantation, considered a state of suspended animation, is widespread in mammals. Blastocysts under this condition remain dormant for an extended period but resume implantation competence upon favorable conditions. The underlying mechanism by which extended longevity of dormant blastocysts is maintained is not clearly understood. Using autophagy markers and the well-defined delayed implantation model in mice, we show that autophagy is important for the extended longevity of dormant blastocysts in utero during delayed implantation. However, prolonged dormancy leads to reduced developmental competency of blastocysts and cellular damage with compromised pregnancy outcome. Estrogen supplementation, which activates implantation of dormant blastocysts, induces the formation of multivesicular bodies in the trophectoderm in vivo. Collectively, our results suggest that autophagy is a critical cellular mechanism that is utilized for the prolonged survival of dormant blastocysts.
Volume 152(5)
Pages 2067-75
Published 2011-5
DOI 10.1210/en.2010-1456
PII en.2010-1456
PMID 21363932
MeSH Adenine / analogs & derivatives Adenine / pharmacology Animals Autophagy / drug effects Autophagy / physiology* Blastocyst / metabolism* Blastocyst / ultrastructure Cell Survival / drug effects Embryo Implantation / physiology* Embryo, Mammalian / cytology Embryo, Mammalian / embryology Embryo, Mammalian / metabolism* Estrogens / pharmacology Female Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Male Mice Mice, Transgenic Microscopy, Confocal Microscopy, Electron, Transmission Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Multivesicular Bodies / metabolism Multivesicular Bodies / ultrastructure Pregnancy Time Factors
IF 3.961
Times Cited 29
Mice GFP-LC3#53