RRC ID 11892
Author Wei X, Xu J, Guo H, Jiang L, Chen S, Yu C, Zhou Z, Hu P, Zhai H, Wan J.
Title DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously.
Journal Plant Physiol.
Abstract The three most important agronomic traits of rice (Oryza sativa), yield, plant height, and flowering time, are controlled by many quantitative trait loci (QTLs). In this study, a newly identified QTL, DTH8 (QTL for days to heading on chromosome 8), was found to regulate these three traits in rice. Map-based cloning reveals that DTH8 encodes a putative HAP3 subunit of the CCAAT-box-binding transcription factor and the complementary experiment increased significantly days to heading, plant height, and number of grains per panicle in CSSL61 (a chromosome segment substitution line that carries the nonfunctional DTH8 allele) with the Asominori functional DTH8 allele under long-day conditions. DTH8 is expressed in most tissues and its protein is localized to the nucleus exclusively. The quantitative real-time PCR assay revealed that DTH8 could down-regulate the transcriptions of Ehd1 (for Early heading date1) and Hd3a (for Heading date3a; a rice ortholog of FLOWERING LOCUS T) under long-day conditions. Ehd1 and Hd3a can also be down-regulated by the photoperiodic flowering genes Ghd7 and Hd1 (a rice ortholog of CONSTANS). Meanwhile, the transcription of DTH8 has been proved to be independent of Ghd7 and Hd1, and the natural mutation of this gene caused weak photoperiod sensitivity and shorter plant height. Taken together, these data indicate that DTH8 probably plays an important role in the signal network of photoperiodic flowering as a novel suppressor as well as in the regulation of plant height and yield potential.
Volume 153(4)
Pages 1747-58
Published 2010-8
DOI 10.1104/pp.110.156943
PII pp.110.156943
PMID 20566706
PMC PMC2923886
MeSH Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Flowers / genetics Flowers / growth & development* Flowers / metabolism Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Mutation Oryza / genetics* Oryza / growth & development Oryza / metabolism Photoperiod Plant Proteins / genetics Plant Proteins / metabolism* Quantitative Trait Loci RNA, Plant / genetics Transcription Factors / genetics Transcription Factors / metabolism*
IF 5.949
Times Cited 154
WOS Category PLANT SCIENCES
Resource
Rice RIL CSSL