論文 - 詳細
| RRC ID | 1190 |
|---|---|
| 著者 | Wasa M, Wang HS, Okada A. |
| タイトル | Characterization of L-glutamine transport by a human neuroblastoma cell line. |
| ジャーナル | Am J Physiol Cell Physiol |
| Abstract |
This study characterized the Na+-dependent transport of L-glutamine by a human neuroblastoma cell line, SK-N-SH. The Na+-dependent component represented >95% of the total glutamine uptake. Kinetic studies showed a single saturable high-affinity carrier with a Michaelis constant (K(m)) of 163 +/- 23 microM and a maximum transport velocity (Vmax) of 13,713 +/- 803 pmol x mg protein(-1) x min(-1). Glutamine uptake was markedly inhibited in the presence of L-alanine, L-asparagine, and L-serine. Li+ did not substitute for Na+. These data show that L-glutamine is predominantly taken up through system ASC. Glutamine deprivation resulted in the decrease of glutamine transport by a mechanism that decreased Vmax without affecting K(m). The expression of the system ASC subtype ASCT2 decreased in the glutamine-deprived group, whereas glutamine deprivation did not induce changes in system ASC subtype ASCT1 mRNA expression. Adaptive increases in Na+-dependent glutamate, Na+-dependent 2-(methylamino)isobutyric acid, and Na+-independent leucine transport were observed under glutamine-deprived conditions, which were completely blocked by actinomycin D and cycloheximide. These mechanisms may allow cells to survive and even grow under nutrient-deprived conditions. |
| 巻・号 | 282(6) |
| ページ | C1246-53 |
| 公開日 | 2002-6-1 |
| DOI | 10.1152/ajpcell.00324.2001 |
| PMID | 11997238 |
| MeSH | Amino Acid Transport System ASC / genetics Amino Acid Transport System ASC / metabolism Amino Acids / pharmacology Biological Transport / drug effects Biological Transport / physiology Cell Division / drug effects Cell Membrane / metabolism Choline / pharmacology Extracellular Space / metabolism Glutamine / metabolism* Glutamine / pharmacokinetics Glutamine / pharmacology Humans Hydrogen-Ion Concentration Lithium / pharmacology Minor Histocompatibility Antigens Neuroblastoma / metabolism* Nucleic Acid Synthesis Inhibitors / pharmacology Protein Synthesis Inhibitors / pharmacology RNA, Messenger / metabolism Sodium / metabolism Tumor Cells, Cultured |
| IF | 3.485 |
| 引用数 | 28 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SK-N-SH(RCB0426) |