RRC ID 11946
Author Yuasa K, Uehara S, Nagahama M, Tsuji A.
Title Transcriptional regulation of cGMP-dependent protein kinase II (cGK-II) in chondrocytes.
Journal Biosci. Biotechnol. Biochem.
Abstract The C-type natriuretic peptide/natriuretic peptide receptor-B/cGMP pathway plays an important role in the regulation of endochondral ossification. In chondrocytes, the physiological effect of cGMP is mediated primarily by the activation of cGMP-dependent protein kinase II (cGK-II). In this study, we investigated the transcriptional regulation of cGK-II in chondrocytes. The expression pattern of cGK-II transcripts was examined during chondrogenic differentiation of ATDC5 cells. cGK-II mRNA was not detectable in undifferentiated cells, but increased dramatically prior to differentiation to the hypertrophic stage. To analyze the transcriptional regulation of cGK-II, the 5'-flanking region of the mouse cGK-II gene was isolated and characterized. The promoter activity of the cGK-II gene decreased markedly following deletion and mutagenesis of the putative Nkx-binding site between nucleotide positions -292 and -286. These results suggest that the homeobox gene Nkx family is critical for the transcriptional regulation of cGK-II during chondrogenesis.
Volume 74(1)
Pages 44-9
Published 2010
DOI 10.1271/bbb.90529
PII JST.JSTAGE/bbb/90529
PMID 20057151
MeSH Animals Base Sequence Cartilage / cytology Cartilage / metabolism Cartilage / physiology Cell Differentiation / genetics Cell Line, Tumor Chondrocytes / metabolism* Chondrogenesis / genetics Cyclic GMP-Dependent Protein Kinase Type II Cyclic GMP-Dependent Protein Kinases / genetics* Gene Expression Regulation, Enzymologic Mice Molecular Sequence Data Promoter Regions, Genetic / genetics Rats Transcription, Genetic*
IF 1.255
Times Cited 3
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material MSM Mouse BAC (RDB04214)