| RRC ID |
1195
|
| 著者 |
Nakajima K, Sonoda H, Mizoguchi T, Aoki J, Arai H, Nagahama M, Tagaya M, Tani K.
|
| タイトル |
A novel phospholipase A1 with sequence homology to a mammalian Sec23p-interacting protein, p125.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
p125, a mammalian Sec23p-interacting protein, exhibits sequence homology with bovine testis phosphatidic acid-preferring phospholipase A(1). In this study, we identified and characterized a new homologue of p125, KIAA0725p. KIAA0725p exhibited remarkable sequence similarity with p125 throughout the entire sequence determined but lacked an N-terminal proline-rich, Sec23p-interacting region. In vitro binding analysis showed that KIAA0725p does not bind to Sec23p. KIAA0725p possessed phospholipase A(1) activity preferentially for phosphatidic acid. We examined the effects of overexpression of KIAA0725p on the morphology of organelles. Overexpression of KIAA0725p, like that of p125, caused dispersion of the endoplasmic reticulum-Golgi intermediate compartment and Golgi apparatus. Different from the case of p125, overexpression of KIAA0725p resulted in dispersion of tethering proteins located in the Golgi region and caused aggregation of the endoplasmic reticulum. Our results indicate that KIAA0725p is a new member of the phosphatidic acid-preferring phospholipase A(1) protein family and suggest that the cellular function of KIAA0725p is different from that of p125.
|
| 巻・号 |
277(13)
|
| ページ |
11329-35
|
| 公開日 |
2002-3-29
|
| DOI |
10.1074/jbc.M111092200
|
| PII |
S0021-9258(18)52159-1
|
| PMID |
11788596
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins / chemistry
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cattle
Chlorocebus aethiops
Cloning, Molecular
DNA Primers
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed
Phospholipases A / chemistry
Phospholipases A / metabolism*
Phospholipases A1
Protein Binding
RNA-Binding Proteins
Sequence Homology, Amino Acid
Subcellular Fractions / metabolism
Vero Cells
|
| IF |
4.238
|
| 引用数 |
72
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Vero(RCB0001) |
