| RRC ID |
1198
|
| Author |
Takayama Y, Mizumachi K, Takezawa T.
|
| Title |
The bovine lactoferrin region responsible for promoting the collagen gel contractile activity of human fibroblasts.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
We have reported that bovine lactoferrin (bLf) promotes the contractile activity of collagen gels by WI-38 human fibroblasts via the phosphorylation of myosin light chain (MLC). To identify the region of bLf that is responsible for this activity, we prepared bLf fragments by limited proteolysis using trypsin and investigated the effects of each fragment on gel contractile activity. Lf consists of a single polypeptide chain containing two lobes that are independent globular structures termed the N- and C-lobes. The fragment corresponding to the C-lobe of bLf (amino acids 341-689) had a more prominent effect on collagen gel contractile activity than did that of either native bLf or its N-lobe (1-284). Further hydrolysis of the C-lobe with either pepsin or trypsin resulted in a loss of this activity. The effect of the C-lobe on collagen gel contraction by fibroblasts was dose-dependent and was associated with the elevation of MLC phosphorylation.
|
| Volume |
299(5)
|
| Pages |
813-7
|
| Published |
2002-12-20
|
| DOI |
10.1016/s0006-291x(02)02748-1
|
| PII |
S0006291X02027481
|
| PMID |
12470651
|
| MeSH |
Animals
Cattle
Cell Line
Collagen / physiology*
Dose-Response Relationship, Drug
Fibroblasts / physiology*
Gels
Humans
Lactoferrin / chemistry*
Lactoferrin / pharmacology*
Myosin Light Chains / metabolism
Peptide Fragments / pharmacology
Phosphorylation
|
| IF |
2.985
|
| Times Cited |
13
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
WI-38 |
