| RRC ID |
1200
|
| 著者 |
Sakuta K, Morihata H, Mori H, Sakai H, Kuno M.
|
| タイトル |
Cell swelling as an intermediate signal leading to activation of a Cl- channel in extracellular calcium-sensing of murine osteoclasts.
|
| ジャーナル |
Osaka City Med J
|
| Abstract |
Osteoclasts possess extracellular Ca2+-sensing machinery to regulate bone-resorbing activity. In murine osteoclasts, an elevation of the extracellular Ca2+ concentration activated a volume-sensitive outwardly rectifying Cl- (OR(Cl)) channel. Exposure to 40 mM Ca2+ activated the OR(Cl) current in association with an increase in the planar cell area. An actin-destabilizer (cytochalasin D), removal of a major extracellular osmolyte (Na+), and a blocker for the Na+/Ca2+ exchanger (2'4'-dichlorobenzamil hydrochloride) inhibited both swelling and activation of the OR(Cl) channel by extracellular Ca2+. There was a positive correlation between the current density and increment in the cell area. The extracellular Ca2+-induced swelling was confirmed in intact (unclamped) cells by three-dimensional analysis using confocal scanning microscopy with a fluorescent dye (BCECF) in the extracellular medium. These results suggest that swelling is an intermediate signal for extracellular Ca2+ sensing of osteoclasts, which leads to activation of the OR(Cl) channel. Cell volume may be a second-message in the regulation of osteoclast function.
|
| 巻・号 |
48(1)
|
| ページ |
29-38
|
| 公開日 |
2002-6-1
|
| PMID |
12375695
|
| MeSH |
Animals
Calcium / metabolism*
Cell Size / physiology*
Chloride Channels / physiology*
Hypotonic Solutions / pharmacology
Male
Mice
Mice, Inbred C3H
Microscopy, Confocal
Osteoclasts / metabolism*
Receptors, Calcium-Sensing
Receptors, Cell Surface / physiology*
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
