| RRC ID |
12021
|
| 著者 |
Tschopp P, Fraudeau N, Béna F, Duboule D.
|
| タイトル |
Reshuffling genomic landscapes to study the regulatory evolution of Hox gene clusters.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
The emergence of Vertebrata was accompanied by two rounds of whole-genome duplications. This enabled paralogous genes to acquire novel functions with high evolutionary potential, a process suggested to occur mostly by changes in gene regulation, rather than in protein sequences. In the case of Hox gene clusters, such duplications favored the appearance of distinct global regulations. To assess the impact of such "regulatory evolution" upon neo-functionalization, we developed PANTHERE (PAN-genomic Translocation for Heterologous Enhancer RE-shuffling) to bring the entire megabase-scale HoxD regulatory landscape in front of the HoxC gene cluster via a targeted translocation in vivo. At this chimeric locus, Hoxc genes could both interpret this foreign regulation and functionally substitute for their Hoxd counterparts. Our results emphasize the importance of evolving regulatory modules rather than their target genes in the process of neo-functionalization and offer a genetic tool to study the complexity of the vertebrate regulatory genome.
|
| 巻・号 |
108(26)
|
| ページ |
10632-7
|
| 公開日 |
2011-6-28
|
| DOI |
10.1073/pnas.1102985108
|
| PII |
1102985108
|
| PMID |
21670281
|
| PMC |
PMC3127922
|
| MeSH |
Animals
Base Sequence
DNA Primers
Evolution, Molecular*
Gene Expression Regulation
Genes, Homeobox*
Genomics*
In Situ Hybridization, Fluorescence
Mice
Multigene Family*
Transcription, Genetic
|
| IF |
9.412
|
| 引用数 |
12
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00805 |
