論文 - 詳細
| RRC ID | 12029 |
|---|---|
| 著者 | Hamaji Y, Fujimori M, Sasaki T, Matsuhashi H, Matsui-Seki K, Shimatani-Shibata Y, Kano Y, Amano J, Taniguchi S. |
| タイトル | Strong enhancement of recombinant cytosine deaminase activity in Bifidobacterium longum for tumor-targeting enzyme/prodrug therapy. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
In our previous studies, a strain of the nonpathogenic, anaerobic, intestinal bacterium, Bifidobacterium longum (B. longum), was found to be localized selectively and to proliferate within solid tumors after systemic administration. In addition, B. longum transformed with the shuttle-plasmid encoding the cytosine deaminase (CD) gene expressed active CD, which deaminated the prodrug 5-fluorocytosine (5-FC) to the anticancer agent 5-fluorouracil (5-FU). We also reported antitumor efficacy with the same plasmid in several animal experiments. In this study, we constructed a novel shuttle-plasmid, pAV001-HU-eCD-M968, which included the mutant CD gene with a mutation at the active site to increase the enzymatic activity. In addition, the plasmid-transformed B. longum produces mutant CD and strongly increased (by 10-fold) its 5-FC to 5-FU enzymatic activity. The use of B. longum harboring the new shuttle-plasmid increases the effectiveness of our enzyme/prodrug strategy. |
| 巻・号 | 71(4) |
| ページ | 874-83 |
| 公開日 | 2007-4-1 |
| DOI | 10.1271/bbb.60502 |
| PII | JST.JSTAGE/bbb/60502 |
| PMID | 17420581 |
| MeSH | Antimetabolites / metabolism* Antineoplastic Agents / chemistry* Antineoplastic Agents / pharmacology* Bifidobacterium / enzymology* Bifidobacterium / genetics Binding Sites / genetics Blotting, Western Chromatography, High Pressure Liquid Cytosine Deaminase / chemistry* Cytosine Deaminase / genetics Cytosine Deaminase / pharmacology* Flucytosine / metabolism* Fluorouracil / metabolism* Mutation / genetics Plasmids / genetics Prodrugs / chemistry* Prodrugs / pharmacology* Recombinant Proteins / chemistry Recombinant Proteins / pharmacology |
| IF | 1.516 |
| 引用数 | 21 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pAxCACD pAdex1CACD (RDB1591) |