RRC ID 12048
Author Naiki T, Nagaki M, Shidoji Y, Kojima H, Moriwaki H.
Title Functional activity of human hepatoma cells transfected with adenovirus-mediated hepatocyte nuclear factor (HNF)-4 gene.
Journal Cell Transplant
Abstract Fulminant hepatic failure (FHF) is still associated with high mortality despite recent advances in medical management. There is need of an effective and safe bioartificial liver (BAL) support to help keep patients with FHF alive until an organ becomes available for transplantation or the native liver recovers. The aim of this study was to establish highly functional liver cells by means of transfecting hepatocyte nuclear factor (HNF)-4 gene for the development of BAL. We constructed adenovirus vector carrying rat HNF-4 cDNA, and transfected to hepatoma-derived cell lines, HepG2 and HuH-7, to enforce expression of the exogenous HNF-4 gene. We analyzed expression of HNF-4, HNF-1, and liver-specific genes in cells infected by the adenovirus vector expressing HNF-4. Adenovirus-mediated HNF-4 gene transfer resulted in increases in expressions of HNF-4, HNF-1, and liver-specific genes such as apolipoproteins, alpha1-antitrypsin (alpha1-AT), phosphoenolpyruvate carboxy-kinase, cytochrome P450 families, and glutamine synthetase in transfected hepatoma cells. Cells overexpressing HNF-4 removed ammonia from medium supplemented with NH4Cl to a greater extent than control cells. These findings demonstrated that transfected cell lines restored differentiated gene expressions and liver-specific function by the overproduction of HNF-4. HNF-4-overexpressing hepatocyte cell lines are useful for bioreactor of BAL systems.
Volume 13(4)
Pages 393-403
Published 2004-1-1
DOI 10.3727/000000004783983855
PMID 15468681
MeSH Adenoviridae / genetics Animals Carcinoma, Hepatocellular Cell Line, Tumor / enzymology Cell Line, Tumor / metabolism* Hepatoblastoma Hepatocyte Nuclear Factor 4 / biosynthesis Hepatocyte Nuclear Factor 4 / genetics* Humans Liver / cytology Liver / enzymology Liver / metabolism Liver, Artificial Rats Transfection
IF 3.341
Times Cited 20
DNA material AxCALacZ Adex1CAlacZ (RDB02726)
Human and Animal Cells Hep G2