RRC ID 12051
Author Kai E, Ochiya T.
Title A method for oral DNA delivery with N-acetylated chitosan.
Journal Pharm Res
Abstract PURPOSE:The gastrointestinal tract poses a variety of morphological and physiological barriers to the expression of a target gene. In this work, N-acetylated chitosan is used as a gene delivery carrier for solving this problem.
METHODS:Plasmid DNAs carrying the lacZ gene and interluekin-10 (IL-10) gene were mixed with N-acetylated chitosan. The N-acetylated chitosan/plasmid DNA complex was mixed into a food paste to feed mice. The transport and distribution characteristics of the plasmid along the intestinal mucosa were identified by beta-galactosidase assay. In addition, the stomach and intestines were subjected to analysis for the production of IL-10.
RESULTS:The efficiency of N-acetylated chitosan-mediated gene delivery to the intestines was observed to be higher than that of chitosan alone. In particular, this result was most significant in the case of the duodenum, where the LacZ gene was expressed most effectively through the use of N-acetylated chitosan. It was also demonstrated that the IL-10 gene was successfully transferred to intestines through this method.
CONCLUSIONS:A plasmid DNA was able to be orally delivered to the intestines using N-acetylated chitosan as a carrier. Thus, we have developed a dietary dose system for delivering a DNA vaccine for treating gastrointestinal diseases.
Volume 21(5)
Pages 838-43
Published 2004-5-1
DOI 10.1023/b:pham.0000026437.32238.6f
PMID 15180343
MeSH Acetylation Animals Chitosan / chemistry* DNA / administration & dosage* Drug Delivery Systems Excipients Gene Transfer Techniques* Interleukin-10 / genetics Intestines / pathology Lac Operon Mice Mice, Inbred C57BL Plasmids / genetics Transgenes beta-Galactosidase / metabolism
IF 3.242
Times Cited 40
DNA material pBluescript SKII+mIL10 (RDB01476)