| RRC ID |
12059
|
| Author |
Nakamura T, Sasaki T, Fujimori M, Yazawa K, Kano Y, Amano J, Taniguchi S.
|
| Title |
Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.
|
| Volume |
66(11)
|
| Pages |
2362-6
|
| Published |
2002-11-1
|
| DOI |
10.1271/bbb.66.2362
|
| PMID |
12506973
|
| MeSH |
Antimetabolites, Antineoplastic / pharmacokinetics*
Antimetabolites, Antineoplastic / therapeutic use
Bifidobacterium / enzymology*
Bifidobacterium / genetics*
Bifidobacterium / metabolism
Blotting, Western
Cloning, Molecular
Cytosine Deaminase
Flucytosine / pharmacokinetics*
Flucytosine / therapeutic use
Fluorouracil / pharmacokinetics*
Fluorouracil / therapeutic use
Gene Expression
Genetic Therapy / methods*
Genetic Vectors / genetics
Hypoxia / physiopathology
Neoplasms / drug therapy
Nucleoside Deaminases / genetics*
Nucleoside Deaminases / metabolism*
Prodrugs / pharmacokinetics*
Prodrugs / therapeutic use
Transfection
Transformation, Bacterial
|
| IF |
1.516
|
| Times Cited |
49
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pAxCACD pAdex1CACD (RDB1591) |
