RRC ID 12059
Author Nakamura T, Sasaki T, Fujimori M, Yazawa K, Kano Y, Amano J, Taniguchi S.
Title Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors.
Journal Biosci. Biotechnol. Biochem.
Abstract Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.
Volume 66(11)
Pages 2362-6
Published 2002-11
PMID 12506973
MeSH Antimetabolites, Antineoplastic / pharmacokinetics* Antimetabolites, Antineoplastic / therapeutic use Bifidobacterium / enzymology* Bifidobacterium / genetics* Bifidobacterium / metabolism Blotting, Western Cloning, Molecular Cytosine Deaminase Flucytosine / pharmacokinetics* Flucytosine / therapeutic use Fluorouracil / pharmacokinetics* Fluorouracil / therapeutic use Gene Expression Genetic Therapy / methods* Genetic Vectors / genetics Hypoxia / physiopathology Neoplasms / drug therapy Nucleoside Deaminases / genetics* Nucleoside Deaminases / metabolism* Prodrugs / pharmacokinetics* Prodrugs / therapeutic use Transfection Transformation, Bacterial
IF 1.255
Times Cited 41
WOS Category CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pAxCACD pAdex1CACD (RDB1591)