論文 - 詳細
| RRC ID | 1206 |
|---|---|
| 著者 | Suzuki T, Futaki S, Niwa M, Tanaka S, Ueda K, Sugiura Y. |
| タイトル | Possible existence of common internalization mechanisms among arginine-rich peptides. |
| ジャーナル | J Biol Chem |
| Abstract |
Basic peptides such as human immunodeficiency virus type 1 (HIV-1) Tat-(48-60) and Drosophila Antennapedia-(43-58) have been reported to have a membrane permeability and a carrier function for intracellular protein delivery. We have shown that not only Tat-(48-60) but many arginine-rich peptides, including HIV-1 Rev-(34-50) and octaarginine (Arg(8)), efficiently translocated through the cell membranes and worked as protein carriers (Futaki, S., Suzuki, T., Ohashi, W., Yagami, T., Tanaka, S., Ueda, K., and Sugiura, Y. (2001) J. Biol. Chem. 276, 5836-5840). Quantification and time course analyses of the cellular uptake of the above peptides by mouse macrophage RAW264.7, human cervical carcinoma HeLa, and simian kidney COS-7 cells revealed that Rev-(34-50) and Arg(8) had a comparable translocation efficiency to Tat-(48-60). Internalization of Tat-(48-60) and Rev-(34-50) was saturable and inhibited by the excess addition of the other peptide. Typical endocytosis and metabolic inhibitors had little effect on the internalization. The uptake of these peptides was significantly inhibited in the presence of heparan sulfate or chondroitin sulfates A, B, and C. Treatment of the cells with the anti-heparan sulfate antibody or heparinase III also lowered the translocation of these peptides. These results strongly suggest that the arginine-rich basic peptides share a certain part of the internalization pathway. |
| 巻・号 | 277(4) |
| ページ | 2437-43 |
| 公開日 | 2002-1-25 |
| DOI | 10.1074/jbc.M110017200 |
| PII | S0021-9258(20)87688-1 |
| PMID | 11711547 |
| MeSH | Amino Acid Sequence Animals Arginine / chemistry* Binding, Competitive COS Cells Cell Line Cell Membrane / metabolism Chondroitin Sulfates / pharmacology Contrast Media / pharmacology Dose-Response Relationship, Drug Drosophila / metabolism Endocytosis / physiology Fluorescein / pharmacology HeLa Cells Heparitin Sulfate / pharmacology Humans L-Lactate Dehydrogenase / metabolism Mice Molecular Sequence Data Peptides / chemistry* Peptides / pharmacology Polysaccharides / pharmacology Protein Structure, Tertiary Protein Transport Time Factors |
| IF | 4.238 |
| 引用数 | 398 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 98 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |