| RRC ID |
1207
|
| Author |
Hashida K, Sakakura Y, Makino N.
|
| Title |
Kinetic studies on the hydrogen peroxide elimination by cultured PC12 cells: rate limitation by glucose-6-phosphate dehydrogenase.
|
| Journal |
Biochim Biophys Acta
|
| Abstract |
Oxidative stress is implicated in the pathogenesis of neurodegenerative disorders and brain ischemia, and hydrogen peroxide (H(2)O(2)) plays a central role in the stress. In this study, we have examined the kinetics of H(2)O(2) elimination by PC12 cells as a neuronal model in connection with the enzyme activities supporting the reaction. Similarly to other cell lines previously studied, H(2)O(2) removal kinetics could be divided into two reactions: one apparently following the Michaelis-Menten kinetics (GSH-dependent reaction) and the other following the first-order kinetics (mainly catalyzed by catalase). Based on the enzyme activities in the cell homogenate, it was inferred that glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme in the GSH- and NADPH-dependent H(2)O(2) elimination by PC12 cells. This is in contrast with fibroblasts and endothelial cells previously examined, in which glutathione reductase (GR) is rate-limiting in the reaction sequence. Treatment of PC12 cells with nerve growth factor increased G6PD activity in the cell homogenate and H(2)O(2) removal activity of the whole cells, with a concomitant increase in the resistance against H(2)O(2) toxicity. These results suggest the importance of G6PD in the antioxidant function of brain and pathogenesis of the oxidative stress-related diseases.
|
| Volume |
1572(1)
|
| Pages |
85-90
|
| Published |
2002-8-15
|
| DOI |
10.1016/s0304-4165(02)00282-9
|
| PII |
S0304416502002829
|
| PMID |
12204336
|
| MeSH |
Animals
Catalase / metabolism
Cell Survival
Cells, Cultured
Dose-Response Relationship, Drug
Glucosephosphate Dehydrogenase / chemistry
Glucosephosphate Dehydrogenase / metabolism*
Glutathione Peroxidase / metabolism
Glutathione Reductase / metabolism
Hydrogen Peroxide / analysis
Hydrogen Peroxide / metabolism*
Hydrogen Peroxide / toxicity
Kinetics
PC12 Cells / chemistry
PC12 Cells / drug effects
PC12 Cells / metabolism*
Rats
|
| IF |
3.411
|
| Times Cited |
25
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
