RRC ID 12080
Author Bae YK, Shimizu T, Hibi M.
Title Patterning of proneuronal and inter-proneuronal domains by hairy- and enhancer of split-related genes in zebrafish neuroectoderm.
Journal Development
Abstract In teleosts and amphibians, the proneuronal domains, which give rise to primary-motor, primary-inter and Rohon-Beard (RB) neurons, are established at the beginning of neurogenesis as three longitudinal stripes along the anteroposterior axis in the dorsal ectoderm. The proneuronal domains are prefigured by the expression of basic helix-loop-helix (bHLH) proneural genes, and separated by domains (inter-proneuronal domains) that do not express the proneural genes. Little is known about how the formation of these domains is spatially regulated. We have found that the zebrafish hairy- and enhancer of split-related (Her) genes her3 and her9 are expressed in the inter-proneuronal domains, and are required for their formation. her3 and her9 expression was not regulated by Notch signaling, but rather controlled by positional cues, in which Bmp signaling is involved. Inhibition of Her3 or Her9 by antisense morpholino oligonucleotides led to ectopic expression of the proneural genes in part of the inter-proneuronal domains. Combined inhibition of Her3 and Her9 induced ubiquitous expression of proneural and neuronal genes in the neural plate, and abolished the formation of the inter-proneuronal domains. Furthermore, inhibition of Her3/Her9 and Notch signaling led to ubiquitous and homogeneous expression of proneural and neuronal genes in the neural plate, revealing that Her3/Her9 and Notch signaling have distinct roles in neurogenesis. These data indicate that her3 and her9 function as prepattern genes that link the positional dorsoventral polarity information in the posterior neuroectoderm to the spatial regulation of neurogenesis.
Volume 132(6)
Pages 1375-85
Published 2005-3
DOI 10.1242/dev.01710
PII dev.01710
PMID 15716337
MeSH Animals Basic Helix-Loop-Helix Transcription Factors Body Patterning / physiology Bone Morphogenetic Proteins / metabolism Ectoderm / metabolism* Membrane Proteins / metabolism Nervous System / metabolism* Neurons / metabolism Receptors, Notch Repressor Proteins / genetics Repressor Proteins / metabolism* Zebrafish / embryology* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism*
IF 5.763
Times Cited 46
Zebrafish ?