RRC ID 12081
Author Taylor MR, Kikkawa S, Diez-Juan A, Ramamurthy V, Kawakami K, Carmeliet P, Brockerhoff SE.
Title The zebrafish pob gene encodes a novel protein required for survival of red cone photoreceptor cells.
Journal Genetics
Abstract The zebrafish mutant, partial optokinetic response b (pob), was isolated using an N-ethyl N-nitrosourea (ENU)-based screening strategy designed to identify larvae with defective optokinetic responses in red but not white light. Previous studies showed that red-light blindness in pob is due to the specific loss of long-wavelength photoreceptor cells via an apoptotic mechanism. Here, we used positional cloning to identify the mutated pob gene. We find that pob encodes a highly conserved 30-kDa protein of unknown function. To demonstrate that the correct gene was isolated, we used the Tol2 transposon system to generate transgenic animals and rescue the mutant phenotype. The Pob protein contains putative transmembrane regions and protein-sorting signals. It is localized to the inner segment and synapse in photoreceptor cells, and when expressed in COS-7 cells it localizes to intracellular compartments. We also show that the degeneration of red cone photoreceptors in the mutants occurs independently of light. On the basis of our findings, we propose that Pob is not involved in phototransduction but rather plays an essential role in protein sorting and/or trafficking.
Volume 170(1)
Pages 263-73
Published 2005-5
DOI 10.1534/genetics.104.036434
PII genetics.104.036434
PMID 15716502
PMC PMC1449739
MeSH Amino Acid Sequence Animals COS Cells Cell Survival / genetics Cercopithecus aethiops Humans Immunohistochemistry In Situ Hybridization Membrane Proteins / genetics* Membrane Proteins / physiology Mice Molecular Sequence Data Retinal Cone Photoreceptor Cells / metabolism* Zebrafish / genetics* Zebrafish / metabolism Zebrafish Proteins / genetics* Zebrafish Proteins / physiology
IF 3.564
Times Cited 20
Zebrafish Method