論文 - 詳細
| RRC ID | 12100 |
|---|---|
| 著者 | Stedman A, Lecaudey V, Havis E, Anselme I, Wassef M, Gilardi-Hebenstreit P, Schneider-Maunoury S. |
| タイトル | A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3. |
| ジャーナル | Dev Biol |
| Abstract |
Patterning of the vertebrate hindbrain involves a segmentation process leading to the formation of seven rhombomeres along the antero-posterior axis. While recent studies have shed light on the mechanisms underlying progressive subdivision of the posterior hindbrain into individual rhombomeres, the early events involved in anterior hindbrain patterning are still largely unknown. In this paper we demonstrate that two zebrafish Iroquois transcription factors, Irx7 and Irx1b, are required for the proper formation and specification of rhombomeres 1 to 4 and, in particular, for krox20 activation in r3. We also show that Irx7 functionally interacts with Meis factors to activate the expression of anterior hindbrain markers, such as hoxb1a, hoxa2 and krox20, ectopically in the anterior neural plate. Then, focusing on krox20 expression, we show that the effect of Irx7 and Meis1.1 is mediated by element C, a conserved cis-regulatory element involved in krox20 activation in the hindbrain. Together, our data point to an essential function of Iroquois transcription factors in krox20 activation and, more generally, in anterior hindbrain specification. |
| 巻・号 | 327(2) |
| ページ | 566-77 |
| 公開日 | 2009-3-15 |
| DOI | 10.1016/j.ydbio.2008.12.018 |
| PII | S0012-1606(08)01451-6 |
| PMID | 19152797 |
| MeSH | Animals Biomarkers / metabolism Body Patterning / physiology* Early Growth Response Protein 2 / genetics Early Growth Response Protein 2 / metabolism* Gene Expression Regulation, Developmental Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* In Situ Hybridization Myeloid Ecotropic Viral Integration Site 1 Protein Neural Plate / anatomy & histology Neural Plate / physiology Regulatory Elements, Transcriptional Rhombencephalon* / anatomy & histology Rhombencephalon* / embryology Transcription Factors / genetics Transcription Factors / metabolism* Zebrafish* / anatomy & histology Zebrafish* / embryology Zebrafish* / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 2.896 |
| 引用数 | 25 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ゼブラフィッシュ | rw0 (Tg(CM-isl1:GFP)) Tol2 method |