RRC ID 12176
Author Kobayashi N, Fujiwara T, Westerman KA, Inoue Y, Sakaguchi M, Noguchi H, Miyazaki M, Cai J, Tanaka N, Fox IJ, Leboulch P.
Title Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes.
Journal Science
Abstract Because of a critical shortage in suitable organs, many patients with terminal liver disease die each year before liver transplantation can be performed. Transplantation of isolated hepatocytes has been proposed for the temporary metabolic support of patients awaiting liver transplantation or spontaneous reversion of their liver disease. A major limitation of this form of therapy is the present inability to isolate an adequate number of transplantable hepatocytes. A highly differentiated cell line, NKNT-3, was generated by retroviral transfer in normal primary adult human hepatocytes of an immortalizing gene that can be subsequently and completely excised by Cre/Lox site-specific recombination. When transplanted into the spleen of rats under transient immunosuppression, reversibly immortalized NKNT-3 cells provided life-saving metabolic support during acute liver failure induced by 90% hepatectomy.
Volume 287(5456)
Pages 1258-62
Published 2000-2-18
DOI 10.1126/science.287.5456.1258
PII 8282
PMID 10678831
MeSH Adult Animals Antigens, Polyomavirus Transforming / genetics Cell Culture Techniques / methods* Cell Differentiation Cell Line Cell Transplantation* Gene Expression Genetic Vectors Hepatectomy Humans Integrases / metabolism Liver / cytology* Liver / metabolism Liver / pathology Liver Failure, Acute / metabolism Liver Failure, Acute / pathology Liver Failure, Acute / prevention & control* Liver Failure, Acute / therapy Liver Regeneration Mice Mice, SCID Rats Retroviridae / genetics Spleen / cytology Transfection Viral Proteins*
IF 41.846
Times Cited 241
DNA material AxCANCre (RDB01748) AxCALacZ Adex1CAlacZ (RDB02726)