論文 - 詳細
| RRC ID | 12191 |
|---|---|
| 著者 | Tatebe S, Matsuura T, Endo K, Teramachi K, Nakamura T, Sato K, Ito H. |
| タイトル | Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells. |
| ジャーナル | Int J Oncol |
| Abstract |
We examined the susceptibility of six human gastric carcinoma cell lines to infection with recombinant p53 adenovirus vector (AxCA-p53). AxCA-p53 infection at a muliplicity of infection (MOI) of 50 resulted in apoptotic cell death (MKN-1 cells), growth arrest (MKN-45, MKN-74 and KATO-III cells), or non-effectiveness (TMK-1 and OCUM-2M cells). Western blot analysis revealed increasing expression levels of p21/WAF1 protein after infection with AxCA-p53 in all the cell lines. After infection with AxCA-p53, the expression levels of bax or bcl-XL protein changed in MKN-1, but not in the other cell lines. These results suggest that the apoptotic pathway (dependence on the expressions of bcl-2 family proteins) dominates the growth arrest pathway (dependence on the expressions of p21/WAF1 protein) after infection with AxCA-p53. Thus, the bcl-2 family might play a crucial role in p53-mediated growth arrest and apoptosis in human gastric carcinoma cells. |
| 巻・号 | 15(2) |
| ページ | 229-35 |
| 公開日 | 1999-8-1 |
| DOI | 10.3892/ijo.15.2.229 |
| PMID | 10402231 |
| MeSH | Adenoviridae / genetics* Apoptosis / physiology* Cell Cycle / physiology Cell Division / physiology DNA Fragmentation Gene Transfer Techniques* Genes, p53* Genetic Vectors* Humans Proto-Oncogene Proteins c-bcl-2 / biosynthesis Recombination, Genetic Stomach Neoplasms / pathology Stomach Neoplasms / therapy* Tumor Cells, Cultured |
| IF | 3.899 |
| 引用数 | 15 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | AxCALacZ Adex1CAlacZ (RDB02726) |