論文 - 詳細
| RRC ID | 1220 |
|---|---|
| 著者 | Takeuchi T, Liang SB, Matsuyoshi N, Zhou S, Miyachi Y, Sonobe H, Ohtsuki Y. |
| タイトル | Loss of T-cadherin (CDH13, H-cadherin) expression in cutaneous squamous cell carcinoma. |
| ジャーナル | Lab Invest |
| Abstract |
We previously reported that T-cadherin (CDH13, H-cadherin), a unique cadherin molecule, was expressed on the basal cell layer in normal murine and human epidermis. In the present study, T-cadherin expression in archival human skin specimens comprising a spectrum of human squamous cell neoplasia was investigated. T-cadherin expression was observed in both normal epidermal basal cells and adnexal epithelial cells of formalin-fixed and paraffin-embedded tissue sections. Western immunoblotting also revealed that mature T-cadherin protein was expressed in cultured human skin tissue equivalent. Atypical keratinocytes in 27 of 53 specimens of actinic keratosis and 23 of 30 specimens of Bowen's disease expressed T-cadherin. In contrast, T-cadherin was focally expressed in 6 of 56 invasive cutaneous squamous cell carcinomas. To explore the molecular mechanism of down-regulation of T-cadherin expression in invasive squamous cell carcinoma, loss of heterozygosity, genetic alternations, and methylation status in the 5' region of the T-cadherin gene were investigated. Loss of heterozygosity at intron 1 of the T-cadherin gene was observed in 8 of 28 informative cases of invasive squamous cell carcinoma. Although no structural genomic alternations were found by sequence analysis, aberrant promoter methylation of the T-cadherin gene was found in 12 of 28 invasive squamous cell carcinomas. T-cadherin expression was restored in cultured A431 cells, in which aberrant methylation was found by treatment with the demethylating agent 5'-aza-2-deoxycytidine. These findings suggest that a combination of deletion and aberrant methylation of the T-cadherin gene may play a role in loss of gene expression in a considerable number of invasive cutaneous squamous cell carcinomas. |
| 巻・号 | 82(8) |
| ページ | 1023-9 |
| 公開日 | 2002-8-1 |
| DOI | 10.1097/01.lab.0000025391.35798.f1 |
| PMID | 12177241 |
| MeSH | Cadherins / biosynthesis* Cadherins / genetics Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism* DNA Methylation Down-Regulation Gene Deletion Humans Skin Neoplasms / genetics Skin Neoplasms / metabolism* |
| IF | 4.197 |
| 引用数 | 56 |
| WOS 分野 | PATHOLOGY MEDICINE, RESEARCH & EXPERIMENTAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | DJM-1(RCB0736) A431(RCB0202) |