RRC ID 12201
Author Yamakuni T, Yamamoto T, Hoshino M, Song SY, Yamamoto H, Kunikata-Sumitomo M, Minegishi A, Kubota M, Ito M, Konishi S.
Title A novel protein containing Cdc10/SWI6 motifs regulates expression of mRNA encoding catecholamine biosynthesizing enzymes.
Journal J Biol Chem
Abstract Catecholaminergic (dopaminergic, noradrenergic, and adrenergic) transmitter phenotypes require the cooperative actions of four biosynthetic enzymes: tyrosine hydroxylase, aromatic L-amino acid decarboxylase, dopamine beta-hydroxylase, and phenylethanolamine N-methyltransferase. Mechanisms that control expression of these enzymes in a transmitter phenotype-specific manner, however, are poorly understood. Here, we provide evidence that overexpression of a novel cdc10/SWI6 motif-containing protein, V-1, elicits the coordinate up-regulation of tyrosine hydroxylase, aromatic L-amino acid decarboxylase, and dopamine beta-hydroxylase mRNAs in the neuronal cell line PC12D, and as a result, catecholamine levels are increased. Furthermore, V-1 is strongly expressed in the cytoplasm of rat chromaffin cells of adrenal medulla. Thus, V-1 may act as a cytoplasmic protein/protein adapter and be involved in control of the catecholaminergic phenotype expression via an intracellular pathway signaling to the nucleus.
Volume 273(42)
Pages 27051-4
Published 1998-10-16
DOI 10.1074/jbc.273.42.27051
PII S0021-9258(19)59635-1
PMID 9765218
MeSH Adrenal Medulla / cytology Adrenal Medulla / metabolism Animals Ankyrin Repeat Aromatic-L-Amino-Acid Decarboxylases / biosynthesis Catecholamines / biosynthesis* Cell Cycle Proteins Chromaffin Cells / cytology Chromaffin Cells / metabolism Cytoskeletal Proteins Dopamine beta-Hydroxylase / biosynthesis GTP Phosphohydrolases Gene Expression Regulation, Enzymologic* Intercellular Signaling Peptides and Proteins* Nerve Tissue Proteins / metabolism* Neurons / metabolism* PC12 Cells Rats Repetitive Sequences, Amino Acid Schizosaccharomyces pombe Proteins Septins Transcription Factors Tyrosine 3-Monooxygenase / biosynthesis Up-Regulation
IF 4.238
Times Cited 30
DNA material pHTH1 Human tyrosine hydroxylase type I cDNA (RDB01269) pPCDC13 EcoA Human aromatic L-amino acid decarboxylase cDNA (RDB01270) pDBH-1 Human dopamine beta-hydroxylase cDNA (RDB01271)