論文 - 詳細
RRC ID | 12201 |
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著者 | Yamakuni T, Yamamoto T, Hoshino M, Song SY, Yamamoto H, Kunikata-Sumitomo M, Minegishi A, Kubota M, Ito M, Konishi S. |
タイトル | A novel protein containing Cdc10/SWI6 motifs regulates expression of mRNA encoding catecholamine biosynthesizing enzymes. |
ジャーナル | J Biol Chem |
Abstract |
Catecholaminergic (dopaminergic, noradrenergic, and adrenergic) transmitter phenotypes require the cooperative actions of four biosynthetic enzymes: tyrosine hydroxylase, aromatic L-amino acid decarboxylase, dopamine beta-hydroxylase, and phenylethanolamine N-methyltransferase. Mechanisms that control expression of these enzymes in a transmitter phenotype-specific manner, however, are poorly understood. Here, we provide evidence that overexpression of a novel cdc10/SWI6 motif-containing protein, V-1, elicits the coordinate up-regulation of tyrosine hydroxylase, aromatic L-amino acid decarboxylase, and dopamine beta-hydroxylase mRNAs in the neuronal cell line PC12D, and as a result, catecholamine levels are increased. Furthermore, V-1 is strongly expressed in the cytoplasm of rat chromaffin cells of adrenal medulla. Thus, V-1 may act as a cytoplasmic protein/protein adapter and be involved in control of the catecholaminergic phenotype expression via an intracellular pathway signaling to the nucleus. |
巻・号 | 273(42) |
ページ | 27051-4 |
公開日 | 1998-10-16 |
DOI | 10.1074/jbc.273.42.27051 |
PII | S0021-9258(19)59635-1 |
PMID | 9765218 |
MeSH | Adrenal Medulla / cytology Adrenal Medulla / metabolism Animals Ankyrin Repeat Aromatic-L-Amino-Acid Decarboxylases / biosynthesis Catecholamines / biosynthesis* Cell Cycle Proteins Chromaffin Cells / cytology Chromaffin Cells / metabolism Cytoskeletal Proteins Dopamine beta-Hydroxylase / biosynthesis GTP Phosphohydrolases Gene Expression Regulation, Enzymologic* Intercellular Signaling Peptides and Proteins* Nerve Tissue Proteins / metabolism* Neurons / metabolism* PC12 Cells Rats Repetitive Sequences, Amino Acid Schizosaccharomyces pombe Proteins Septins Transcription Factors Tyrosine 3-Monooxygenase / biosynthesis Up-Regulation |
IF | 4.238 |
引用数 | 30 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
遺伝子材料 | pHTH1 Human tyrosine hydroxylase type I cDNA (RDB01269) pPCDC13 EcoA Human aromatic L-amino acid decarboxylase cDNA (RDB01270) pDBH-1 Human dopamine beta-hydroxylase cDNA (RDB01271) |