RRC ID 12202
Author Takahashi S, Takahashi Y, Yoshimi T, Miura T.
Title Oxygen tension regulates heme oxygenase-1 gene expression in mammalian cell lines.
Journal Cell Biochem Funct
Abstract The gene expression of heme oxygenase-1 (HO-1) was studied in mammalian cell lines exposed to hyperoxia. Northern blot analysis demonstrated that hyperoxic exposure increased the HO-1 mRNA levels in various types of cells, including human hepatoma (HepG2) cells. This increase was time- and dose-dependent, and reversible. The HO-1 mRNA levels in HepG2 cells were increased to 2.3- and 4.2-fold of the control by hyperoxic exposure of 6 and 23 h, respectively. Cycloheximide and actinomycin D inhibited the increases in the HO-1 mRNA level produced by hyperoxia, indicating that response to hyperoxia is dependent on de novo protein synthesis and mRNA transcription. Antioxidants, desferrioxamine (DES) and o-phenanthroline (OP) partially inhibited the HO-1 mRNA elevation by hyperoxia. In addition to hyperoxia, sodium arsenite (NaAsO2), cadmium chloride (CdCl(2)) and hydrogen peroxide (H2O2), which are reactive oxygen intermediates (ROI) generators, increased the HO-1 mRNA level by 11-, 22- and 2.5-fold, respectively. OP, an antioxidant and a bivalent metal chelator, blocked the HO-1 mRNA elevation induced either by hyperoxia or by the three ROI generators. In contrast to OP, N-acetylcysteine (NAC), an antioxidant and membrane-permeable reducing reagent, enhanced the HO-1 mRNA elevation induced by hyperoxia, although NAC inhibited the mRNA elevation induced by NaAsO2, CdCl2 and H2O2. These results indicate that oxygen tension regulates HO-1 gene expression and suggest that hyperoxia-specific and redox-sensitive regulators may be involved in hyperoxia-mediated HO-1 gene expression.
Volume 16(3)
Pages 183-93
Published 1998-9-1
DOI 10.1002/(SICI)1099-0844(199809)16:3<183::AID-CBF784>3.0.CO;2-0
PII 10.1002/(SICI)1099-0844(199809)16:3<183::AID-CBF784>3.0.CO;2-0
PMID 9747510
MeSH Animals Antioxidants / pharmacology Cell Line Cell Survival Cycloheximide / pharmacology Dactinomycin / pharmacology Dose-Response Relationship, Drug Gene Expression Regulation, Enzymologic* Heme Oxygenase (Decyclizing) / biosynthesis* Heme Oxygenase (Decyclizing) / genetics Heme Oxygenase-1 Humans Membrane Proteins Mice Nucleic Acid Synthesis Inhibitors / pharmacology Oxidants / pharmacology Oxygen / pharmacology* Protein Synthesis Inhibitors / pharmacology RNA, Messenger / analysis Rats
IF 2.632
Times Cited 22
DNA material pHHO1 Human heme oxygenase cDNA (RDB01207)
Human and Animal Cells Hep G2(RCB0459) NRK(RCB0043)