RRC ID 12212
Author Ohashi T, Sato S, Yoshiki A, Kusakabe M.
Title TSC-36 (follistatin-related polypeptide) gene expression in estrogen receptor positive osteoblastic cell line, CDO7F.
Journal Calcif. Tissue Int.
Abstract Using an osteoblastic cell line (CDO7F) that was selected as an estrogen receptor positive cell by immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) subtractive cDNA cloning using oligo(dT)30-Latex and PCR was performed to isolate a gene that is induced by estrogen. A cDNA clone was isolated and its nucleotide sequence identified it as TSC 36, previously called follistatin-related polypeptide. Northern blot analysis showed that the TSC-36 gene expression was certainly upregulated by estrogen. Tamoxifen also upregulated this gene expression. These findings indicate that TSC-36 may be one of the estrogen-regulated genes in osteoblastic cells.
Volume 61(5)
Pages 400-3
Published 1997-11
PMID 9351882
MeSH Animals Cell Line Cloning, Molecular DNA, Complementary / genetics Estrogen Antagonists / pharmacology Estrogens / pharmacology Follistatin-Related Proteins Gene Expression Regulation / genetics* Glycoproteins / biosynthesis Glycoproteins / genetics* Mice Osteoblasts / drug effects* Osteoblasts / metabolism Receptors, Estrogen / genetics* Receptors, Estrogen / metabolism Tamoxifen / pharmacology Up-Regulation
IF 3.293
Times Cited 8
DNA material pTGF-11 (RDB01195)