Reference - Detail
|Author||Ohashi T, Sato S, Yoshiki A, Kusakabe M.|
|Title||TSC-36 (follistatin-related polypeptide) gene expression in estrogen receptor positive osteoblastic cell line, CDO7F.|
|Journal||Calcif. Tissue Int.|
Using an osteoblastic cell line (CDO7F) that was selected as an estrogen receptor positive cell by immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) subtractive cDNA cloning using oligo(dT)30-Latex and PCR was performed to isolate a gene that is induced by estrogen. A cDNA clone was isolated and its nucleotide sequence identified it as TSC 36, previously called follistatin-related polypeptide. Northern blot analysis showed that the TSC-36 gene expression was certainly upregulated by estrogen. Tamoxifen also upregulated this gene expression. These findings indicate that TSC-36 may be one of the estrogen-regulated genes in osteoblastic cells.
|MeSH||Animals Cell Line Cloning, Molecular DNA, Complementary / genetics Estrogen Antagonists / pharmacology Estrogens / pharmacology Follistatin-Related Proteins Gene Expression Regulation / genetics* Glycoproteins / biosynthesis Glycoproteins / genetics* Mice Osteoblasts / drug effects* Osteoblasts / metabolism Receptors, Estrogen / genetics* Receptors, Estrogen / metabolism Tamoxifen / pharmacology Up-Regulation|
|WOS Category||ENDOCRINOLOGY & METABOLISM|
|DNA material||pTGF-11 (RDB01195)|