RRC ID 12213
Author Rokutan K, Teshima S, Miyoshi M, Nikawa T, Kishi K.
Title Oxidant-induced activation of nuclear factor-kappa B in cultured guinea pig gastric epithelial cells.
Journal Dig Dis Sci
Abstract The aim of this study was to reveal oxidant-sensitive components in gastric epithelial cells, which may regulate inflammatory processes in gastric mucosa. Gel mobility shift assay showed that treatment of cultured guinea pig gastric epithelial cells with hydrogen peroxide or diamide produced a KB oligonucleotide-protein complex within 5 min. The binding proteins consisted of a p50/p65 heterodimer, which was identified by immunosupershift, UV cross-linking, and immunoprecipitation analyses. Immunocytochemical study demonstrated that surface epithelial cells and parietal cells expressed p500 and p65 mainly in the cytosol, and the oxidants rapidly initiated the nuclear translocation of the components. The oxidants caused the up-regulation of p105 (a p50 precursor) synthesis and the expression of inducible nitric oxide synthase mRNA. These results suggest that the oxidant-sensitive p50/p65 heterodimer in gastric epithelial cells may play an important role in transcriptional activation of genes involved in inflammatory responses of the stomach.
Volume 42(9)
Pages 1880-9
Published 1997-9-1
DOI 10.1023/a:1018859026105
PMID 9331151
MeSH Animals Blotting, Northern Blotting, Western Cells, Cultured DNA / metabolism DNA-Binding Proteins / metabolism Diamide / pharmacology Epithelial Cells Gastric Mucosa / cytology Gastric Mucosa / drug effects Gastric Mucosa / immunology Gastric Mucosa / metabolism* Guinea Pigs Hydrogen Peroxide / pharmacology Male NF-kappa B / genetics NF-kappa B / metabolism* Nitric Oxide Synthase / biosynthesis Oxidants / pharmacology* Oxidative Stress RNA, Messenger / genetics Sulfhydryl Reagents / pharmacology Time Factors
IF 2.751
Times Cited 12
DNA material Rat iNOS2-2U (RDB01385)