Reference - Detail
| RRC ID | 12229 |
|---|---|
| Author | Kojima H, Inuzuka S, Miwa T, Furukawa S, Hayashi K, Kanegae Y, Saito I, Ohishi N, Takamori M, Yagi K. |
| Title | Construction and characterization of adenoviral vector expressing biologically active brain-derived neurotrophic factor. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
To deliver brain-derived neurotrophic factor (BDNF) to the central nervous system, we sought to attain adenovirus-mediated transfer and expression of the gene in both in vitro and in vivo experiments. For this purpose, we constructed AxCA-BDNF, a recombinant adenoviral vector containing the BDNF cDNA expression cassette. Reverse transcription polymerase chain reaction analyses of the infected HeLa cells and the transduced mouse brain revealed successful expression of the BDNF gene both in vitro and in vivo. The results of a survival assay of chick dorsal root ganglion cells showed that the produced BDNF was biologically active. We consider, therefore, this newly constructed recombinant adenovirus to be a useful tool to deliver BDNF to degenerating neurons and to be applicable to gene therapy of neurodegenerative diseases and nerve trauma. |
| Volume | 212(2) |
| Pages | 712-7 |
| Published | 1995-7-17 |
| DOI | 10.1006/bbrc.1995.2027 |
| PII | S0006-291X(85)72027-X |
| PMID | 7542888 |
| MeSH | Adenoviridae / genetics* Animals Base Sequence Brain / metabolism Brain-Derived Neurotrophic Factor Cell Survival Chickens Culture Media, Conditioned DNA, Complementary / genetics Ganglia, Spinal / cytology Gene Expression Gene Transfer Techniques Genetic Vectors* HeLa Cells Humans Mice Molecular Sequence Data Nerve Growth Factors / genetics Nerve Tissue Proteins / genetics* Polymerase Chain Reaction RNA, Messenger / metabolism RNA-Directed DNA Polymerase Recombinant Proteins / biosynthesis |
| IF | 2.985 |
| Times Cited | 13 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pAxcw pAdex1cw (RDB00917) |