論文 - 詳細
| RRC ID | 12230 |
|---|---|
| 著者 | Wada M, Abe K, Okumura K, Taguchi H, Kohno K, Imamoto F, Schlessinger D, Kuwano M. |
| タイトル | Chimeric YACs were generated at unreduced rates in conditions that suppress coligation. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Chimerism is a major limitation of current YAC libraries. A method based on partially filled-in ends of restriction fragments was designed to avoid coligation as a possible source of chimeras. Model experiments using plasmid DNA as an insert showed that coligation was clearly avoided by this method. Pilot collections of YACs with an average insert size of 650kb were then constructed with and without the partial fill-in treatment. Starting from a mixture of a equal amounts of human and mouse DNA, none of 108 clones was positive by hybridization with both Alu and B2 probes, again suggesting that coligation was effectively blocked. However, 4 out of 10 clones still hybridized to 2 or more locations by FISH on chromosomes in human metaphase spreads, level similar to that in the clones made without the partial fill-in step. These results strongly suggest that chimeric clones generally arise by a mechanism independent of coligation, presumptively based on recombination. |
| 巻・号 | 22(9) |
| ページ | 1651-4 |
| 公開日 | 1994-5-11 |
| DOI | 10.1093/nar/22.9.1651 |
| PMID | 8202367 |
| PMC | PMC308044 |
| MeSH | Animals Cell Line Chromosomes, Artificial, Yeast* DNA, Recombinant Gene Library Humans Kinetics Mice Plasmids Sepharose |
| IF | 11.502 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pBLUR8 (RDB01029) |