RRC ID 12231
著者 Sugiyama D, Kulkeaw K, Mizuochi C, Horio Y, Okayama S.
タイトル Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production.
ジャーナル Biochem Biophys Res Commun
Abstract In mammals, definitive erythropoiesis first occurs in fetal liver (FL), although little is known about how the process is regulated. FL consists of hepatoblasts, sinusoid endothelial cells and hematopoietic cells. To determine niche cells for fetal liver erythropoiesis, we isolated each FL component by flow cytometry. mRNA analysis suggested that Dlk-1-expressing hepatoblasts primarily expressed EPO and SCF, genes encoding erythropoietic cytokines. EPO protein was detected predominantly in hepatoblasts, as assessed by ELISA and immunohistochemistry, and was not detected in sinusoid endothelial cells and hematopoietic cells. To characterize hepatoblast function in FL, we analyzed Map2k4(-/-) mouse embryos, which lack hepatoblasts, and observed down-regulation of EPO and SCF expression in FL relative to wild-type mice. Our observations demonstrate that hepatoblasts comprise a niche for erythropoiesis through cytokine secretion.
巻・号 410(2)
ページ 301-6
公開日 2011-7-1
DOI 10.1016/j.bbrc.2011.05.137
PII S0006-291X(11)00919-3
PMID 21664343
MeSH Animals Cytokines / biosynthesis* Down-Regulation Erythropoiesis* Fetus / physiology* Flow Cytometry Hematopoietic Stem Cells / physiology* Liver / embryology* MAP Kinase Kinase 4 / genetics Mice Mice, Inbred C57BL Mice, Inbred ICR
IF 2.985
引用数 22
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
実験動物マウス RBRC00438