| RRC ID |
12234
|
| 著者 |
Nakayama M, Kaida A, Deguchi S, Sakaguchi K, Miura M.
|
| タイトル |
Radiosensitivity of early and late M-phase HeLa cells isolated by a combination of fluorescent ubiquitination-based cell cycle indicator (Fucci) and mitotic shake-off.
|
| ジャーナル |
Radiat Res
|
| Abstract |
The mitotic shake-off method revealed the remarkable variation of radiosensitivity of HeLa cells during the cell cycle: M phase shows the greatest radiosensitivity and late S phase the greatest radioresistance. This method harvests all M-phase cells with a round shape, making it impossible to further subdivide M-phase cells. Recently, the fluorescent ubiquitination-based cell cycle indicator (Fucci) was developed; this system basically causes cells in G(1) to emit red fluorescence and other cells to emit green fluorescence. Because the green fluorescence rapidly disappears at late M phase, two-dimensional flow cytometry analysis can usually detect a green(high)/red(low) fraction including S-, G(2)- and early M-phase cells but not a transitional fraction between green(high)/red(low) and green(low)/red(low) including late M-phase cells. However, combining the shake-off method concentrated the transitional fraction, which enabled us to separate early and late M-phase cells without using any drugs. Here we demonstrate for the first time that cells in early M phase are more radiosensitive than those in late M phase, implying that early M phase is the most radiosensitive sub-phase during the cell cycle.
|
| 巻・号 |
176(3)
|
| ページ |
407-11
|
| 公開日 |
2011-9-1
|
| DOI |
10.1667/rr2608.1
|
| PII |
10.1667/RR2608.1
|
| PMID |
21692656
|
| MeSH |
Cell Division*
Fluorescence
HeLa Cells / radiation effects*
Humans
Mitosis*
Radiation Tolerance*
Ubiquitination
|
| IF |
2.657
|
| 引用数 |
6
|
|
WOS 分野
|
BIOLOGY
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOPHYSICS
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa/Fucci(RCB2812) |
