RRC ID 12295
Author St-Onge R, Gadkar VJ, Arseneault T, Goyer C, Filion M.
Title The ability of Pseudomonas sp. LBUM 223 to produce phenazine-1-carboxylic acid affects the growth of Streptomyces scabies, the expression of thaxtomin biosynthesis genes and the biological control potential against common scab of potato.
Journal FEMS Microbiol Ecol
Abstract Streptomyces scabies causes common scab, an economical disease affecting potato crops world-wide, for which no effective control measure exists. This pathogen produces the plant toxin thaxtomin A, which is involved in symptom development on potato tubers. A biological control approach that can limit S. scabies growth and repress thaxtomin production represents an attractive alternative to classical control strategies. Pseudomonas sp. LBUM 223 produces phenazine-1-carboxylic acid (PCA), an antibiotic that inhibits the growth of plant pathogens and contributes to the biological control of plant diseases. In this study, the involvement of LBUM 223's PCA-producing ability in the growth inhibition of S. scabies, repression of thaxtomin biosynthesis genes (txtA and txtC) and the biological control of common scab of potato was investigated using a mutant defective in PCA production (LBUM 223phzC(-) ). Streptomyces scabies growth was inhibited to a significantly lesser degree by LBUM 223phzC(-) than by the wild type. LBUM 223 also significantly repressed txtA and txtC expression in S. scabies and protected potato against disease, whereas LBUM 223phzC(-) did not. These results suggest that PCA production is central to the ability of LBUM 223 to limit pathogen growth, repress the expression of key pathogenicity genes and control common scab of potato.
Volume 75(1)
Pages 173-83
Published 2011-1-1
DOI 10.1111/j.1574-6941.2010.00992.x
PMID 21073487
MeSH Antibiosis Gene Expression Regulation, Bacterial Genes, Bacterial Indoles / metabolism* Mutation Phenazines / metabolism Piperazines / metabolism* Plant Diseases / microbiology* Plant Diseases / prevention & control Pseudomonas / genetics Pseudomonas / metabolism* Pseudomonas / pathogenicity Solanum tuberosum / microbiology* Streptomyces / genetics Streptomyces / growth & development* Streptomyces / metabolism Streptomyces / pathogenicity
IF 3.675
Times Cited 33
Prokaryotes E. coli E.coli strain S17-1(Lambdapir)