| RRC ID |
123
|
| 著者 |
Yamate J, Yokoyama Y, Kumagi D, Tsukamoto Y, Kuwamura M, Kotani T, Sakuma S.
|
| タイトル |
Tumour lines from a spontaneous rat endometrial stromal sarcoma, showing dendritic cell-like and myofibroblastic cell-like phenotypes.
|
| ジャーナル |
J Comp Pathol
|
| Abstract |
A transplantable tumour (RY) and cell lines (RY-PB and clone RY-B-E3 isolated from RY-PB) were established from a naturally occurring endometrial stromal sarcoma (ESS) found in a 24-month-old female F344 rat. The primary tumour and RY tumours, which had been serially passaged in syngeneic female rats up to the 10th generation, consisted of spindle or round cells arranged in ill-defined bundles or sheets. Neoplastic cells of the primary and RY tumours, as well as cultured cells of RY-PB and RY-B-E3, showed positive reactions to vimentin, ED1/ED2 (both for rat macrophages/histiocytes), OX6 (for dendritic cells expressing rat MHC class II antigens), and lysosomal enzymes such as acid phosphatase and non-specific esterase, in varying degrees. Ultrastructurally, neoplastic cells characteristically had tubulovesicular system-like structures and variously developed lysosomes in the cytoplasm. Neoplastic cells also exhibited immunoexpression to an alpha-smooth muscle actin (alpha-SMA). The addition of transforming growth factor (TGF)-beta1 to RY-PB and RY-B-E3 cultures increased the number of alpha-SMA-positive cells, whilst the positive cell number was decreased by anti-TGF-beta antibody. The RT-PCR method revealed the expression of TGF-beta1 mRNA in the cultured cells. The present study showed that rat ESS-derived cells exhibited dendritic cell-like and myofibroblastic cell-like phenotypes. The histogenesis of ESSs in human beings and rats remains poorly understood, and these tumour lines may therefore become useful tools for further research.
|
| 巻・号 |
131(1)
|
| ページ |
38-51
|
| 公開日 |
2004-7-1
|
| DOI |
10.1016/j.jcpa.2004.01.003
|
| PII |
S0021997504000064
|
| PMID |
15144798
|
| MeSH |
Animals
Apoptosis / physiology
Cell Line, Tumor*
Cell Transplantation
DNA Fragmentation
Dendritic Cells / metabolism
Dendritic Cells / pathology
Endometrial Neoplasms / metabolism
Endometrial Neoplasms / pathology*
Female
Fibroblasts / metabolism
Fibroblasts / pathology
Immunohistochemistry
Microscopy, Electron, Transmission
Neoplasm Transplantation
RNA, Messenger / analysis
Rats
Rats, Inbred F344
Reverse Transcriptase Polymerase Chain Reaction
Sarcoma, Endometrial Stromal / metabolism
Sarcoma, Endometrial Stromal / pathology*
Sarcoma, Experimental / metabolism
Sarcoma, Experimental / pathology*
Transforming Growth Factor beta / biosynthesis
Transforming Growth Factor beta1
|
| IF |
0.994
|
| 引用数 |
4
|
|
WOS 分野
|
VETERINARY SCIENCES
PATHOLOGY
|
| リソース情報 |
| ラット |
|
