RRC ID 123
Author Yamate J, Yokoyama Y, Kumagi D, Tsukamoto Y, Kuwamura M, Kotani T, Sakuma S.
Title Tumour lines from a spontaneous rat endometrial stromal sarcoma, showing dendritic cell-like and myofibroblastic cell-like phenotypes.
Journal J. Comp. Pathol.
Abstract A transplantable tumour (RY) and cell lines (RY-PB and clone RY-B-E3 isolated from RY-PB) were established from a naturally occurring endometrial stromal sarcoma (ESS) found in a 24-month-old female F344 rat. The primary tumour and RY tumours, which had been serially passaged in syngeneic female rats up to the 10th generation, consisted of spindle or round cells arranged in ill-defined bundles or sheets. Neoplastic cells of the primary and RY tumours, as well as cultured cells of RY-PB and RY-B-E3, showed positive reactions to vimentin, ED1/ED2 (both for rat macrophages/histiocytes), OX6 (for dendritic cells expressing rat MHC class II antigens), and lysosomal enzymes such as acid phosphatase and non-specific esterase, in varying degrees. Ultrastructurally, neoplastic cells characteristically had tubulovesicular system-like structures and variously developed lysosomes in the cytoplasm. Neoplastic cells also exhibited immunoexpression to an alpha-smooth muscle actin (alpha-SMA). The addition of transforming growth factor (TGF)-beta1 to RY-PB and RY-B-E3 cultures increased the number of alpha-SMA-positive cells, whilst the positive cell number was decreased by anti-TGF-beta antibody. The RT-PCR method revealed the expression of TGF-beta1 mRNA in the cultured cells. The present study showed that rat ESS-derived cells exhibited dendritic cell-like and myofibroblastic cell-like phenotypes. The histogenesis of ESSs in human beings and rats remains poorly understood, and these tumour lines may therefore become useful tools for further research.
Volume 131(1)
Pages 38-51
Published 2004-7
DOI 10.1016/j.jcpa.2004.01.003
PII S0021997504000064
PMID 15144798
MeSH Animals Apoptosis / physiology Cell Line, Tumor* Cell Transplantation DNA Fragmentation Dendritic Cells / metabolism Dendritic Cells / pathology Endometrial Neoplasms / metabolism Endometrial Neoplasms / pathology* Female Fibroblasts / metabolism Fibroblasts / pathology Immunohistochemistry Microscopy, Electron, Transmission Neoplasm Transplantation RNA, Messenger / analysis Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction Sarcoma, Endometrial Stromal / metabolism Sarcoma, Endometrial Stromal / pathology* Sarcoma, Experimental / metabolism Sarcoma, Experimental / pathology* Transforming Growth Factor beta / biosynthesis Transforming Growth Factor beta1
IF 1.364
Times Cited 4