論文 - 詳細
| RRC ID | 12333 |
|---|---|
| 著者 | Reinhold H, Soyk S, Simková K, Hostettler C, Marafino J, Mainiero S, Vaughan CK, Monroe JD, Zeeman SC. |
| タイトル | β-amylase-like proteins function as transcription factors in Arabidopsis, controlling shoot growth and development. |
| ジャーナル | Plant Cell |
| Abstract |
Plants contain β-amylase-like proteins (BAMs; enzymes usually associated with starch breakdown) present in the nucleus rather than targeted to the chloroplast. They possess BRASSINAZOLE RESISTANT1 (BZR1)-type DNA binding domains--also found in transcription factors mediating brassinosteroid (BR) responses. The two Arabidopsis thaliana BZR1-BAM proteins (BAM7 and BAM8) bind a cis-regulatory element that both contains a G box and resembles a BR-responsive element. In protoplast transactivation assays, these BZR1-BAMs activate gene expression. Structural modeling suggests that the BAM domain's glucan binding cleft is intact, but the recombinant proteins are at least 1000 times less active than chloroplastic β-amylases. Deregulation of BZR1-BAMs (the bam7bam8 double mutant and BAM8-overexpressing plants) causes altered leaf growth and development. Of the genes upregulated in plants overexpressing BAM8 and downregulated in bam7bam8 plants, many carry the cis-regulatory element in their promoters. Many genes that respond to BRs are inversely regulated by BZR1-BAMs. We propose a role for BZR1-BAMs in controlling plant growth and development through crosstalk with BR signaling. Furthermore, we speculate that BZR1-BAMs may transmit metabolic signals by binding a ligand in their BAM domain, although diurnal changes in the concentration of maltose, a candidate ligand produced by chloroplastic β-amylases, do not influence their transcription factor function. |
| 巻・号 | 23(4) |
| ページ | 1391-403 |
| 公開日 | 2011-4-1 |
| DOI | 10.1105/tpc.110.081950 |
| PII | tpc.110.081950 |
| PMID | 21487098 |
| PMC | PMC3101533 |
| MeSH | Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis Proteins / chemistry Arabidopsis Proteins / metabolism* Base Sequence Gene Expression Regulation, Plant Genes, Plant / genetics Glucans / metabolism Hydrolysis Models, Biological Molecular Sequence Data Mutant Proteins / isolation & purification Mutant Proteins / metabolism Mutation / genetics Nuclear Proteins / metabolism Plant Leaves / genetics Plant Leaves / growth & development Plant Shoots / enzymology Plant Shoots / growth & development* Protein Binding Protein Structure, Tertiary Response Elements / genetics Trans-Activators / metabolism Transcription Factors / chemistry Transcription Factors / metabolism* beta-Amylase / chemistry beta-Amylase / metabolism* |
| IF | 9.618 |
| 引用数 | 49 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pdx36441 pdx38715 pdx39980 pdz61236 pdz86189 |