| RRC ID |
12339
|
| Author |
Miyashita M, Oda M, Ono Y, Komoda E, Miyagawa H.
|
| Title |
Discovery of a small peptide from combinatorial libraries that can activate the plant immune system by a jasmonic acid signaling pathway.
|
| Journal |
Chembiochem
|
| Abstract |
Plants defend themselves by using an innate immune system that is activated in response to a variety of molecules derived from pathogens. These molecules have provided profound insights into the mechanisms of pathogen recognition and subsequent signaling pathways in plants. In the present study, we screened a combinatorial random hexapeptide library for peptides that activate the plant immune system, by using a cell-based high-throughput screening system in which H(2)O(2) generation was monitored. We discovered a novel small peptide (YGIHTH-amide, PIP-1) that triggered H(2)O(2) production in tobacco and tomato cells, but not in Arabidopsis cells. PIP-1 induced significant levels of phytoalexin biosynthesis and defense-related gene expression in tobacco cells; this is likely to be activated by a jasmonic acid pathway.
|
| Volume |
12(9)
|
| Pages |
1323-9
|
| Published |
2011-6-14
|
| DOI |
10.1002/cbic.201000694
|
| PMID |
21567702
|
| MeSH |
Amino Acid Sequence
Arabidopsis / chemistry
Arabidopsis / metabolism*
Combinatorial Chemistry Techniques
Cyclopentanes / metabolism*
Hydrogen Peroxide / chemistry
Hydrogen Peroxide / metabolism
Immunity, Innate* / drug effects
Lycopersicon esculentum / chemistry
Molecular Sequence Data
Oligopeptides / chemistry
Oligopeptides / genetics
Oligopeptides / metabolism*
Oligopeptides / pharmacology
Oxylipins / metabolism*
Plant Immunity* / drug effects
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / drug effects
Small Molecule Libraries* / chemistry
Structure-Activity Relationship
Tobacco / chemistry
|
| IF |
2.576
|
| Times Cited |
9
|
|
WOS Category
|
CHEMISTRY, MEDICINAL
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00012 |
