RRC ID 12355
Author Guo T, Liu X, Wan X, Weng J, Liu S, Liu X, Chen M, Li J, Su N, Wu F, Cheng Z, Guo X, Lei C, Wang J, Jiang L, Wan J.
Title Identification of a stable quantitative trait locus for percentage grains with white chalkiness in rice (Oryza sativa).
Journal J Integr Plant Biol
Abstract High chalkiness is a major problem in many rice-producing areas of the world, especially in hybrid rice (Oryza sativa L.) in China. We previously showed a major quantitative trait locus for the percentage of grains with white chalkiness (QTLqPGWC-8) in the interval G1149-R727 on chromosome 8 using a chromosome segment substitution line (CSSL). Here, we selected the line-CSSL50 harboring the QTLqPGWC-8 allele from the CSSLs derived from a cross between Asominori (as a recurrent parent) and IR24 (as a donor parent), which had higher percentage chalkiness, markedly different from that of Asominori. There were also significant differences in starch granules, appearance of amylose content (AAC) and milling qualities between Asominori and CSSL50, but not in grain size or thousand grain weight (TGW). The BC(4) F(2) and BC(4) F(3) populations from a cross between CSSL50 and Asominori were used for fine mapping of qPGWC-8. We narrowed down the location of this QTL to a 142 kb region between Indel markers 8G-7 and 8G-9. QTLqPGWC-8 accounted for 50.9% of the difference in PGWC between the parents. The markers tightly linked to qPGWC-8 should facilitate cloning of the gene underlying this QTL and will be of value for marker-assisted selection in breeding rice varieties with better grain quality.
Volume 53(8)
Pages 598-607
Published 2011-8
DOI 10.1111/j.1744-7909.2011.01041.x
PMID 21518249
MeSH Amylose / metabolism Base Sequence Biomass Chromosome Mapping Genotype Microsatellite Repeats / genetics Molecular Sequence Data Oryza / genetics* Oryza / ultrastructure Phenotype Quantitative Trait Loci / genetics* Quantitative Trait, Heritable* Seeds / genetics* Seeds / ultrastructure Starch / metabolism
IF 3.092
Times Cited 16
WOS Category PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Rice CSSL