論文 - 詳細
| RRC ID | 12376 |
|---|---|
| 著者 | Yamazoe H, Okuyama T, Suzuki H, Fukuda J. |
| タイトル | Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices. |
| ジャーナル | Acta Biomater |
| Abstract |
A surface coated with cross-linked albumin film resists the adhesion of cells, and subsequent exposure to UV irradiation or electrostatic adsorption of a cationic polymer switches the surface from non-adherent to adherent. Taking advantage of this unique property of cross-linked albumin, the authors fabricated patterned cell co-cultures with desired patterns and cell types. In this scheme, the cell-adherent region was initially created in the cell-non-adhesive albumin substrate, on which a first cell type was attached. Subsequently, the remaining region was also changed to adherent for the attachment of secondary cells in the same manner, thereby allowing distinctly localized co-cultures. As a proof of concept demonstration of the feasibility of this approach, a patterned co-culture of Neuro-2a cells with L929 cells was successfully prepared on the substrate. Furthermore, combining this technique with a microfluidic technique, a micropatterned co-culture of PA6 cells with 3T3 fibroblasts was created inside microfluidic devices. This approach could potentially be a useful tool for fundamental investigations of cell-cell interactions and for tissue engineering applications. |
| 巻・号 | 6(2) |
| ページ | 526-33 |
| 公開日 | 2010-2-1 |
| DOI | 10.1016/j.actbio.2009.07.036 |
| PII | S1742-7061(09)00321-3 |
| PMID | 19654059 |
| MeSH | Albumins / metabolism* Coculture Techniques Humans Microfluidics / instrumentation* Microscopy, Electron Spectrometry, Fluorescence Spectrophotometry, Ultraviolet |
| IF | 7.242 |
| 引用数 | 16 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | L929(RCB1451) MC3T3-G2/PA6RCB1127) 3T3 Swiss Albino(RCB1642) |