論文 - 詳細
| RRC ID | 12377 |
|---|---|
| 著者 | Sakai S, Hirose K, Moriyama K, Kawakami K. |
| タイトル | Control of cellular adhesiveness in an alginate-based hydrogel by varying peroxidase and H(2)O(2) concentrations during gelation. |
| ジャーナル | Acta Biomater |
| Abstract |
An aqueous solution of alginate possessing phenolic hydroxyl (Alg-Ph) groups is gellable via a horseradish peroxidase (HRP)-catalyzed oxidative crosslinking reaction between Ph groups, consuming H(2)O(2) as an electron acceptor. This study evaluates the effect of H(2)O(2) and HRP concentrations on cellular adhesiveness and proliferation on the resultant enzymatically crosslinked Alg-Ph gels. After 4h of seeding, 81.1% of L929 fibroblast cells adhere to an Alg-Ph hydrogel prepared with 1 U ml(-1) HRP and 1mM H(2)O(2). Increasing the concentration of H(2)O(2) to 15 mM decreases the percentage of adhering cells to 28.4%. The cellular adhesion at this H(2)O(2) concentration is increased to 82.6% by increasing the HRP concentration to 10 U ml(-1). The cells adhering to the Alg-Ph hydrogels with higher cellular adhesiveness establish a confluent monolayer during 168 h of culture. A cell sheet can then be harvested within 5 min of immersion in a medium containing alginate lyase at 1.0 mg ml(-1). The harvested cell sheet re-adhere, and the cells contained in the sheet proliferate after being transferred to another cell culture dish. |
| 巻・号 | 6(4) |
| ページ | 1446-52 |
| 公開日 | 2010-4-1 |
| DOI | 10.1016/j.actbio.2009.10.004 |
| PII | S1742-7061(09)00438-3 |
| PMID | 19818883 |
| MeSH | Alginates / pharmacology* Animals Biocatalysis / drug effects Cell Adhesion / drug effects Cell Proliferation / drug effects Fibroblasts / cytology Fibroblasts / drug effects Glucuronic Acid / pharmacology Hexuronic Acids / pharmacology Horseradish Peroxidase / pharmacology Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology* Hydrogen Peroxide / pharmacology* Hydroxyl Radical Mechanical Phenomena / drug effects Mice Solutions Time Factors |
| IF | 7.242 |
| 引用数 | 30 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | L929(RCB1451) |