| RRC ID |
12381
|
| 著者 |
Inaba R, Khademhosseini A, Suzuki H, Fukuda J.
|
| タイトル |
Electrochemical desorption of self-assembled monolayers for engineering cellular tissues.
|
| ジャーナル |
Biomaterials
|
| Abstract |
Adherent cells, cell sheets, and spheroids were harvested noninvasively from a culture surface by means of electrochemical desorption of a self-assembled monolayer (SAM) of alkanethiol. The SAM surface was made adhesive by the covalent bonding of Arg-Gly-Asp (RGD)-peptides to the alkanethiol molecules. The application of a negative electrical potential caused the reductive desorption of the SAM, resulting in the detachment of the cells. Using this approach greater than 90% of adherent cells detached within 5 min. Furthermore, this approach was used to obtain two-dimensional (2D) cell sheets. The detached cell sheets consisted of viable cells, which could be easily attached to other cell sheets in succession to form a multilayered cell sheet. Moreover, spheroids of hepatocytes of a uniform diameter were formed in an array of cylindrical cavities at a density of 280 spheroids/cm(2) and were harvested by applying a negative electrical potential. This cell manipulation technology could potentially be a useful tool for the fabrication and assembly of building blocks such as cell sheets and spheroids for regenerative medicine and tissue engineering applications.
|
| 巻・号 |
30(21)
|
| ページ |
3573-9
|
| 公開日 |
2009-7-1
|
| DOI |
10.1016/j.biomaterials.2009.03.045
|
| PII |
S0142-9612(09)00296-8
|
| PMID |
19362363
|
| MeSH |
3T3 Cells
Animals
Cell Adhesion
Electrochemistry / methods*
Mice
Oligopeptides / chemistry
Tissue Engineering / methods
|
| IF |
10.317
|
| 引用数 |
100
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
3T3 Swiss Albino(RCB1642) |
