Reference - Detail
| RRC ID | 12381 |
|---|---|
| Author | Inaba R, Khademhosseini A, Suzuki H, Fukuda J. |
| Title | Electrochemical desorption of self-assembled monolayers for engineering cellular tissues. |
| Journal | Biomaterials |
| Abstract |
Adherent cells, cell sheets, and spheroids were harvested noninvasively from a culture surface by means of electrochemical desorption of a self-assembled monolayer (SAM) of alkanethiol. The SAM surface was made adhesive by the covalent bonding of Arg-Gly-Asp (RGD)-peptides to the alkanethiol molecules. The application of a negative electrical potential caused the reductive desorption of the SAM, resulting in the detachment of the cells. Using this approach greater than 90% of adherent cells detached within 5 min. Furthermore, this approach was used to obtain two-dimensional (2D) cell sheets. The detached cell sheets consisted of viable cells, which could be easily attached to other cell sheets in succession to form a multilayered cell sheet. Moreover, spheroids of hepatocytes of a uniform diameter were formed in an array of cylindrical cavities at a density of 280 spheroids/cm(2) and were harvested by applying a negative electrical potential. This cell manipulation technology could potentially be a useful tool for the fabrication and assembly of building blocks such as cell sheets and spheroids for regenerative medicine and tissue engineering applications. |
| Volume | 30(21) |
| Pages | 3573-9 |
| Published | 2009-7-1 |
| DOI | 10.1016/j.biomaterials.2009.03.045 |
| PII | S0142-9612(09)00296-8 |
| PMID | 19362363 |
| MeSH | 3T3 Cells Animals Cell Adhesion Electrochemistry / methods* Mice Oligopeptides / chemistry Tissue Engineering / methods |
| IF | 10.317 |
| Times Cited | 100 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 3T3 Swiss Albino(RCB1642) |