論文 - 詳細
| RRC ID | 12381 |
|---|---|
| 著者 | Inaba R, Khademhosseini A, Suzuki H, Fukuda J. |
| タイトル | Electrochemical desorption of self-assembled monolayers for engineering cellular tissues. |
| ジャーナル | Biomaterials |
| Abstract |
Adherent cells, cell sheets, and spheroids were harvested noninvasively from a culture surface by means of electrochemical desorption of a self-assembled monolayer (SAM) of alkanethiol. The SAM surface was made adhesive by the covalent bonding of Arg-Gly-Asp (RGD)-peptides to the alkanethiol molecules. The application of a negative electrical potential caused the reductive desorption of the SAM, resulting in the detachment of the cells. Using this approach greater than 90% of adherent cells detached within 5 min. Furthermore, this approach was used to obtain two-dimensional (2D) cell sheets. The detached cell sheets consisted of viable cells, which could be easily attached to other cell sheets in succession to form a multilayered cell sheet. Moreover, spheroids of hepatocytes of a uniform diameter were formed in an array of cylindrical cavities at a density of 280 spheroids/cm(2) and were harvested by applying a negative electrical potential. This cell manipulation technology could potentially be a useful tool for the fabrication and assembly of building blocks such as cell sheets and spheroids for regenerative medicine and tissue engineering applications. |
| 巻・号 | 30(21) |
| ページ | 3573-9 |
| 公開日 | 2009-7-1 |
| DOI | 10.1016/j.biomaterials.2009.03.045 |
| PII | S0142-9612(09)00296-8 |
| PMID | 19362363 |
| MeSH | 3T3 Cells Animals Cell Adhesion Electrochemistry / methods* Mice Oligopeptides / chemistry Tissue Engineering / methods |
| IF | 10.317 |
| 引用数 | 100 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 3T3 Swiss Albino(RCB1642) |