| RRC ID |
12406
|
| Author |
Ishiwata I, Yasuda M, Hirano T, Ishikawa H.
|
| Title |
Establishment and characterization of HEPFT, a cell line derived from hepatoid carcinoma of the fallopian tube, with special reference to alpha-fetoprotein, lectin affinity and histogenesis.
|
| Journal |
Hum Cell
|
| Abstract |
A cell line designated "HEPFT" was established from a human fallopian tubal hepatoid carcinoma. This line grew well without interruption for 13 months and was subcultivated over 35 times. The cells were spherical and polygonal in shape and showed neoplastic and pleomorphic features such as a bizarre aggregation of chromatin granules, an irregular thickening membrane and multiple large nucleoli. The cells formed epithelial colonies with a jigsaw puzzle-like arrangement and multilayering without contact inhibition. The cells contained moderate to abundant amounts of eosinophilic cytoplasm and were immunohistochemically positive for alpha-fetoprotein. The cells proliferated rapidly, and the population doubling time was about 45 h. The chromosome number showed a wide distribution of aneuploidy. The modal chromosome number was stable in the hyper triploid range and many marker chromosomes were observed. The culture cells produced bile and a large amount of lentil lectin-reactive alpha-fetoprotein. The recently developed bacterial artificial chromosome array comparative genomic hybridization facilitated detailed analysis with high resolution and sensitivity. Different profiles of genomic copy-number abnormalities were demonstrated in various chromosomal regions in HEPFT cells.
|
| Volume |
20(4)
|
| Pages |
119-30
|
| Published |
2007-11-1
|
| DOI |
10.1111/j.1749-0774.2007.00039.x
|
| PII |
HUC039
|
| PMID |
17949352
|
| MeSH |
Aged
Aneuploidy
Animals
Carcinoma, Hepatocellular / genetics*
Carcinoma, Hepatocellular / metabolism*
Carcinoma, Hepatocellular / pathology
Cell Line, Tumor
Cystadenocarcinoma, Papillary / metabolism
Cystadenocarcinoma, Papillary / pathology
Fallopian Tube Neoplasms / genetics*
Fallopian Tube Neoplasms / metabolism*
Fallopian Tube Neoplasms / pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Karyotyping
Mice
Mice, Inbred BALB C
Middle Aged
Neoplasm Transplantation
Plant Lectins
Reverse Transcriptase Polymerase Chain Reaction
alpha-Fetoproteins / analysis
alpha-Fetoproteins / biosynthesis*
|
| IF |
3.463
|
| Times Cited |
2
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
SKN(RCB0513) |
