論文 - 詳細
| RRC ID | 12407 |
|---|---|
| 著者 | Okada Y, Okada T, Numata M, Hayashi Y, Yamashima T, Yamashita J. |
| タイトル | Increased expression of deoxyribonucleic acid methyltransferase gene in human astrocytic tumors. |
| ジャーナル | Neurol Med Chir (Tokyo) |
| Abstract |
The relationship between the grade of astrocytic tumor and the expression of deoxyribonucleic acid methyltransferase (DNA-MTase) gene was examined. The levels of DNA-MTase messenger ribonucleic acid (mRNA) were measured by semiquantitative reverse transcriptase-polymerase chain reaction in surgical specimens from 12 astrocytic tumors (4 astrocytomas, 6 anaplastic astrocytomas, and 2 glioblastomas) and two normal brain tissues, and in four glioma cell lines. Compared to normal brain tissues, the levels of DNA-MTase mRNA were increased by 16- to 55-fold in low grade astrocytomas, and significantly increased by 200- to 4500-fold in high grade astrocytomas (anaplastic astrocytomas and glioblastomas) and more than 4500-fold in glioma cell lines. In situ hybridization with paraffin-embedded surgical specimens of human astrocytic tumors showed DNA-MTase mRNA was abundantly expressed in high grade astrocytomas. The detection of increased DNA-MTase expression in astrocytic tumor indicates involvement in the tumorigenesis and suggests that blocking of this change with specific inhibitors may offer new therapeutic strategies for malignant astrocytic tumors. |
| 巻・号 | 40(11) |
| ページ | 564-70; discussion 570-1 |
| 公開日 | 2000-11-1 |
| DOI | 10.2176/nmc.40.564 |
| PII | JST.JSTAGE/nmc/40.564 |
| PMID | 11109793 |
| MeSH | Astrocytoma / genetics* Blotting, Southern Brain Neoplasms / genetics* DNA, Neoplasm / genetics* Gene Expression Regulation, Neoplastic Glioblastoma / genetics Humans In Situ Hybridization In Vitro Techniques Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured tRNA Methyltransferases / genetics* |
| 引用数 | 17 |
| WOS 分野 | RHEUMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | U251(RCB0461) U-87 MG(RCB0419) |