Reference - Detail
| RRC ID | 12414 |
|---|---|
| Author | Nakamura K, Kanno T, Mokudai T, Iwasawa A, Niwano Y, Kohno M. |
| Title | A novel analytical method to evaluate directly catalase activity of microorganisms and mammalian cells by ESR oximetry. |
| Journal | Free Radic Res |
| Abstract |
Electron spin resonance (ESR) oximetry technique was applied for analysis of catalase activity in the present study. Catalase activity was evaluated by measuring oxygen from the reaction between hydrogen peroxide (H(2)O(2)) and catalase-positive cells. It was demonstrated that the ESR spectra of spin-label probes, 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL), 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (4-oxo-TEMPO) and 4-maleimido-2,2,6,6-tetramethyl-1-piperidinyloxy (4-maleimido-TEMPO) in the presence of H(2)O(2) were broadened with the concentrations of catalase. It was possible to make a calibration curve for catalase activity by peak widths of the spectra of each spin-label probe, which are broadened dependently on catalase concentrations. The broadened ESR spectra were also observed when the catalase-positive micro-organisms or the mammalian cells originally from circulating monocytes/macrophages were mixed with TEMPOL and H(2)O(2). Meanwhile, catalase-negative micro-organisms caused no broadening change of ESR spectra. The present study indicates that it is possible to evaluate directly the catalase activity of various micro-organisms and mammalian cells by using an ESR oximetry technique. |
| Volume | 44(9) |
| Pages | 1036-43 |
| Published | 2010-9-1 |
| DOI | 10.3109/10715762.2010.495750 |
| PMID | 20815766 |
| MeSH | Animals Bacillus subtilis / chemistry Bacillus subtilis / metabolism Bacteria / metabolism* Calibration Catalase / analysis Catalase / metabolism* Cells / metabolism Cells, Cultured Chemistry Techniques, Analytical / methods* Cyclic N-Oxides / chemistry Cyclic N-Oxides / pharmacology Electron Spin Resonance Spectroscopy / methods Electron Spin Resonance Spectroscopy / standards Escherichia coli / chemistry Escherichia coli / metabolism Humans Mammals / metabolism* Mice Oxygen / analysis* Spin Labels Triacetoneamine-N-Oxyl / chemistry Triacetoneamine-N-Oxyl / pharmacology |
| IF | 2.839 |
| Times Cited | 2 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |