RRC ID 12416
Author Shirasawa Y, Shibahara-Sone H, Iino T, Ishikawa F.
Title Bifidobacterium bifidum BF-1 suppresses Helicobacter pylori-induced genes in human epithelial cells.
Journal J Dairy Sci
Abstract Helicobacter pylori infection alters gene expression in host cells. Specifically, inflammatory chemokines such as IL-8 are upregulated in the gastric mucosa during H. pylori infection. Although the mechanism by which H. pylori causes inflammation of the gastric mucosa is not yet understood, many studies have suggested that nuclear factor kappa B (NF-κB) plays a key regulatory role in host cells. We have shown that preincubation with Bifidobacterium bifidum strain BF-1, a probiotic strain known to improve H. pylori-associated gastritis, suppresses induction of IL-8 by the pathogen. To investigate how how BF-1 affects gene expression in H. pylori-infected cells, we performed microarray analysis to assess gene expression in epithelial cells, which had been preincubated with BF-1 and infected with H. pylori. We found that preincubation with BF-1 suppresses the expression of H. pylori-induced genes in human cells and that most of the affected genes are related to the NF-κB signaling pathways. These results suggest that BF-1 can affect the regulatory mechanism of the NF-κB signaling pathways.
Volume 93(10)
Pages 4526-34
Published 2010-10-1
DOI 10.3168/jds.2010-3274
PII S0022-0302(10)00483-2
PMID 20854986
MeSH Bifidobacterium / classification Bifidobacterium / metabolism* Cell Culture Techniques Cell Line Epithelial Cells / microbiology* Gastric Mucosa / microbiology* Gene Expression Regulation* Helicobacter pylori / physiology* Humans Interleukin-8 / metabolism NF-kappa B / physiology Probiotics Signal Transduction
IF 3.333
Times Cited 18
Human and Animal Cells GCIY(RCB0555)