RRC ID 12426
Author Kamio N, Akifusa S, Yamaguchi N, Nonaka K, Yamashita Y.
Title Anti-inflammatory activity of a globular adiponectin function on RAW 264 cells stimulated by lipopolysaccharide from Aggregatibacter actinomycetemcomitans.
Journal FEMS Immunol Med Microbiol
Abstract Adiponectin is an adipokine with potent anti-inflammatory properties. We previously reported that a globular adiponectin (gAd) suppresses Aggregatibacter actinomycetemcomitans lipopolysaccharide-induced nuclear factor-kappaB activity, suggesting an anti-inflammatory effect of gAd. In this study, we investigated whether gAd is able to modulate the effect of A. actinomycetemcomitans lipopolysaccharide on cytokine induction in a murine macrophage cell line (RAW 264). The phosphorylation of p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, extracellular signal-regulated kinase, and IkappaB kinase alpha/beta and the degradation of IkappaB, which were induced by A. actinomycetemcomitans lipopolysaccharide intoxication, were clearly reduced in gAd-pretreated RAW 264 cells compared with the untreated cells. Expression levels of tumor necrosis factor (TNF)-alpha and interleukin-10 (IL-10) mRNA were assessed by real-time PCR. Cell-free supernatants were collected after 12 h of stimulation and analyzed by enzyme-linked immunosorbent assay for TNF-alpha and IL-10. Pretreatment with gAd significantly inhibited the A. actinomycetemcomitans lipopolysaccharide-induced TNF-alpha mRNA expression and protein secretion. In contrast, pretreatment with gAd significantly enhanced the A. actinomycetemcomitans lipopolysaccharide-induced IL-10 mRNA expression and protein secretion. These data suggest a mechanism for the anti-inflammatory activity of gAd in local inflammatory lesions, such as periodontitis.
Volume 56(3)
Pages 241-7
Published 2009-8-1
DOI 10.1111/j.1574-695X.2009.00573.x
PMID 19552715
MeSH Adiponectin / immunology Adiponectin / pharmacology Animals Cell Line Enzyme-Linked Immunosorbent Assay / methods Extracellular Signal-Regulated MAP Kinases / metabolism Gene Expression Profiling / methods I-kappa B Kinase / metabolism Immunologic Factors / immunology* Immunologic Factors / pharmacology Interleukin-10 / antagonists & inhibitors* Interleukin-10 / biosynthesis Interleukin-10 / metabolism JNK Mitogen-Activated Protein Kinases / metabolism Lipopolysaccharides / immunology* Lipopolysaccharides / isolation & purification Macrophages / drug effects Macrophages / immunology* Mice Pasteurellaceae / chemistry* Pasteurellaceae / immunology Phosphorylation Tumor Necrosis Factor-alpha / antagonists & inhibitors* Tumor Necrosis Factor-alpha / biosynthesis Tumor Necrosis Factor-alpha / metabolism p38 Mitogen-Activated Protein Kinases / metabolism
Times Cited 18
Human and Animal Cells RAW 264(RCB0535)